Spark NIR™ 685 anti-mouse CD11c antibody, CD11c, N418

Pricing & Availability

Clone: N418 (See other available formats)
Regulatory Status: RUO
Other Names: αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype: Armenian Hamster IgG
Ave. Rating: Submit a Review
Product Citations: publications

C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E FITC and anti-mouse CD11c (clone N418) Spark NIR™ 685 (left), or C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E FITC only (right).

Compare all formats See Spark NIR™ 685 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

117367

25 µg

£97

Check Availability

Need larger quantities of this item?
Request Bulk Quote

117368

100 µg

£257

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

CD11c is a 150 kD glycoprotein also known as α_X integrin, CR4, and p150. CD11c forms a α_Xβ₂ heterodimer with β₂ integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The α_Xβ₂ integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse spleen dendritic cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation³, immunohistochemical staining of acetone-fixed frozen sections³, immunofluorescence microscopy^{5, 9} (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections¹⁰), and spatial biology (IBEX)^22,23.
RRID: AB_2894416 (BioLegend Cat. No. 117367)
AB_2894416 (BioLegend Cat. No. 117368)

Antigen Details

Structure: Integrin α-chain, associates with integrin β₂ (CD18), 150 kD
Distribution: Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function: Cellular adhesion
Ligand/Receptor: iC3b, fibrinogen
Cell Type: Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.
Gene ID: 16411 View all products for this Gene ID
UniProt: View information about CD11c on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11c Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD11c	N418	FC
Biotin anti-mouse CD11c	N418	FC,IHC-F
FITC anti-mouse CD11c	N418	FC
PE anti-mouse CD11c	N418	FC
Purified anti-mouse CD11c	N418	FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c	N418	FC
Alexa Fluor® 647 anti-mouse CD11c	N418	FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c	N418	FC
PE/Cyanine7 anti-mouse CD11c	N418	FC
Brilliant Violet 605™ anti-mouse CD11c	N418	FC
Alexa Fluor® 700 anti-mouse CD11c	N418	FC
Pacific Blue™ anti-mouse CD11c	N418	FC
APC/Cyanine7 anti-mouse CD11c	N418	FC
PerCP/Cyanine5.5 anti-mouse CD11c	N418	FC
PerCP anti-mouse CD11c	N418	FC
Brilliant Violet 421™ anti-mouse CD11c	N418	FC,IHC
Brilliant Violet 570™ anti-mouse CD11c	N418	FC
Brilliant Violet 785™ anti-mouse CD11c	N418	FC
Brilliant Violet 510™ anti-mouse CD11c	N418	FC
Brilliant Violet 650™ anti-mouse CD11c	N418	FC
Purified anti-mouse CD11c (Maxpar® Ready)	N418	FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c	N418	IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c	N418	FC
Brilliant Violet 711™ anti-mouse CD11c	N418	FC
APC/Fire™ 750 anti-mouse CD11c	N418	FC
TotalSeq™-A0106 anti-mouse CD11c	N418	PG
Brilliant Violet 750™ anti-mouse CD11c	N418	FC
TotalSeq™-B0106 anti-mouse CD11c	N418	PG
TotalSeq™-C0106 anti-mouse CD11c	N418	PG
KIRAVIA Blue 520™ anti-mouse CD11c	N418	FC
Spark Blue™ 550 anti-mouse CD11c	N418	FC
Spark NIR™ 685 anti-mouse CD11c	N418	FC
Spark UV™ 387 anti-mouse CD11c	N418	FC
Spark Red™ 718 anti-mouse CD11c	N418	FC
Spark Blue™ 515 anti-mouse CD11c	N418	FC
PerCP/Fire™ 806 anti-mouse CD11c	N418	FC
Spark PLUS UV395™ anti-mouse CD11c	N418	FC
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™)	N418	FC

Certificate of Analysis

Customers Also Purchased

Alexa Fluor® 488 anti-mouse CD49b

Clone: HMα2

Alexa Fluor® 700 anti-mouse I-A/I-E

Clone: M5/114.15.2

APC/Fire™ 750 anti-mouse CD3

Clone: 17A2

PE anti-mouse F4/80

Clone: BM8

Go To Top Version: 1 Revision Date: 07/07/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.