- Clone
- N418 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- αX integrin, integrin αX chain, CR4, p150, ITGAX
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E FITC and anti-mouse CD11c (clone N418) Spark NIR™ 685 (left), or C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E FITC only (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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117367 | 25 µg | £97 | ||||
117368 | 100 µg | £257 |
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse spleen dendritic cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm. - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
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Application References
(PubMed link indicates BioLegend citation) -
- Granucci F, et al. 1997. J. Immunol. 159:1794.
- Stokes RW, et al. 1998. J. Immunol. 160:5514.
- Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
- Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
- Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
- You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
- Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
- Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
- Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
- Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
- Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
- Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
- Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
- Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
- Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
- White CE, et al. 2015. J Immunol. 194:697. PubMed
- Lu X, et al. 2015. J Immunol. 194:2011. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- RRID
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AB_2894416 (BioLegend Cat. No. 117367)
AB_2894416 (BioLegend Cat. No. 117368)
Antigen Details
- Structure
- Integrin α-chain, associates with integrin β2 (CD18), 150 kD
- Distribution
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Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
- Function
- Cellular adhesion
- Ligand/Receptor
- iC3b, fibrinogen
- Cell Type
- Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780. - Gene ID
- 16411 View all products for this Gene ID
- UniProt
- View information about CD11c on UniProt.org
Related FAQs
Other Formats
View All CD11c Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD11c
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Biotin anti-mouse CD11c
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FITC anti-mouse CD11c
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PE anti-mouse CD11c
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Purified anti-mouse CD11c
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Alexa Fluor® 488 anti-mouse CD11c
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Alexa Fluor® 647 anti-mouse CD11c
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PE/Cyanine5 anti-mouse CD11c
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PE/Cyanine7 anti-mouse CD11c
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Brilliant Violet 605™ anti-mouse CD11c
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Alexa Fluor® 700 anti-mouse CD11c
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Pacific Blue™ anti-mouse CD11c
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APC/Cyanine7 anti-mouse CD11c
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PerCP/Cyanine5.5 anti-mouse CD11c
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PerCP anti-mouse CD11c
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Brilliant Violet 421™ anti-mouse CD11c
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Brilliant Violet 570™ anti-mouse CD11c
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Brilliant Violet 785™ anti-mouse CD11c
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Brilliant Violet 510™ anti-mouse CD11c
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Brilliant Violet 650™ anti-mouse CD11c
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Purified anti-mouse CD11c (Maxpar® Ready)
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Alexa Fluor® 594 anti-mouse CD11c
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PE/Dazzle™ 594 anti-mouse CD11c
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Brilliant Violet 711™ anti-mouse CD11c
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APC/Fire™ 750 anti-mouse CD11c
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TotalSeq™-A0106 anti-mouse CD11c
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Brilliant Violet 750™ anti-mouse CD11c
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TotalSeq™-B0106 anti-mouse CD11c
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TotalSeq™-C0106 anti-mouse CD11c
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KIRAVIA Blue 520™ anti-mouse CD11c
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Spark Blue™ 550 anti-mouse CD11c
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Spark NIR™ 685 anti-mouse CD11c
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Spark UV™ 387 anti-mouse CD11c
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Spark Red™ 718 anti-mouse CD11c
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Spark Blue™ 515 anti-mouse CD11c
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