Purified anti-MEK1 Phospho (Ser298) Antibody

Pricing & Availability
Clone
A16117B (See other available formats)
Other Names
MEK1, MAP2K1, CFC3, MKK1, MAPKK1, PRKMK1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16117B_Purified_MEK1_Phospho_Antibody_050719.png
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole overnight were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-MEK1 Phospho (Ser298) Antibody, clone A16117B, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal MEK1 loading was confirmed by probing membranes with an anti-pan MEK1 antibody. Lane M: Molecular Weight marker.
  • A16117B_Purified_MEK1_Phospho_Antibody_050719.png
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole overnight were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-MEK1 Phospho (Ser298) Antibody, clone A16117B, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal MEK1 loading was confirmed by probing membranes with an anti-pan MEK1 antibody. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
610601 25 µg £64
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610602 100 µg £151
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Description

MEK1 and MEK2 (Map/Erk Kinase) are serine/threonine kinases in the MAPK/ERK signaling axis that play a critical role in cellular proliferation, differentiation, and migration. Phosphorylation at MEK1 (Ser298) by PAK1 enhances the ability of upstream Raf kinases to phosphorylate regulatory serines (Ser218 and Ser222) in the activation loop, thereby stimulating kinase activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human MEK1 peptide phosphorylated at Ser298. Complete Freund’s adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
MEK1 is a 393 amino acid protein with a predicted molecular weight of 43 kD
Distribution

Ubiquitously expressed/cytosolic

Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Song C, et al. 2017. Sci Rep. 7:46833.
  2. Jeong DE, et al. 2017. EMBO J. 36:1046.
  3. Kyriakakis E, et al. 2017. Cell Signal. 35:163.
  4. Narbonne P, et al. PLoS Genet. 13:e1006738.
  5. Wu H, et al. 2013. PLoS One. 8:e83737.
  6. Wang Z, et al. 2010. Oncogene. 29:3362.
  7. Moniz S, et al. 2007. Oncogene. 26:6071.
  8. Park ER, et al. 2007. Cell Signal. 7:1488.
Gene ID
5604 View all products for this Gene ID
UniProt
View information about MEK1 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/07/2019

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