PE anti-MEK1 Phospho (Ser298) Antibody

Pricing & Availability
Clone
A16117B (See other available formats)
Other Names
MEK1, MAP2K1, CFC3, MKK1, MAPKK1, PRKMK1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16117B_PE_Phospho_Ser298_Antibody_011519.png
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with anti-MEK1 Phospho (Ser298) Antibody (clone A16117B) PE.
  • A16117B_PE_Phospho_Ser298_Antibody_011519.png
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with anti-MEK1 Phospho (Ser298) Antibody (clone A16117B) PE.
See PE spectral data
Cat # Size Price Quantity Avail. Save
610603 25 tests £84
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610604 100 tests £198
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Description

MEK1 and MEK2 (Map/Erk Kinase) are serine/threonine kinases in the MAPK/ERK signaling axis that play a critical role in cellular proliferation, differentiation, and migration. Phosphorylation at MEK1 (Ser298) by PAK1 enhances ability of upstream Raf kinases to phosphorylate regulatory serines (Ser218 and Ser222) in the activation loop, thereby stimulating kinase activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human MEK1 peptide phosphorylated at Ser298. Complete Freund’s adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
MEK1 is a 393 amino acid protein with a predicted molecular weight of 43 kD
Distribution

Ubiquitously expressed/cytosolic

Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Song C, et al. 2017. Sci Rep. 7:46833.
  2. Jeong DE, et al. 2017. EMBO J. 36:1046.
  3. Kyriakakis E, et al. 2017. Cell Signal. 35:163.
  4. Narbonne P, et al. PLoS Genet. 13:e1006738.
  5. Wu H, et al. 2013. PLoS One. 8:e83737.
  6. Wang Z, et al. 2010. Oncogene. 29:3362.
  7. Moniz S, et al. 2007. Oncogene. 26:6071.
  8. Park ER, et al. 2007. Cell Signal. 7:1488.
Gene ID
5604 View all products for this Gene ID
UniProt
View information about MEK1 Phospho on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/15/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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