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Purified anti-IRAK1 Antibody

Pricing & Availability
Clone
W22166E (See other available formats)
Regulatory Status
RUO
Other Names
AA48924; Il1rak; Interleukin 1 receptor associated kinase 1; IRAK; IRAK-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22166E_PURE_IRAK1_Antibody_1_013124
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-IRAK1 (clone W22166E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22166E_PURE_IRAK1_Antibody_1_013124
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-IRAK1 (clone W22166E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22166E_PURE_IRAK1_Antibody_2_021524
    293T cells were fixed with ice-cold methanol, and then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-IRAK1 (clone W22166E), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panel B). The image was captured with a 40X objective. Scale bar: 25 µm
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632051 25 µg £117
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632052 100 µg £301
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Description

The interleukin-1 receptor-associated kinase (IRAK) family of proteins are made up of serine/threonine-specific kinases which play a critical role in the response to pathogens through the induction of acute inflammation and subsequent adaptive immune responses. The mammalian IRAK molecular family consists of four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). Two are active kinases, IRAK1 and IRAK4, and two are inactive kinases, IRAK2 and IRAKM. However, all are involved in the regulation of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1R1, IL-1RAcP, MyD88, and IRAKs. Currently, there are three differentially spliced variants of IRAK1: IRAK1, IRAK1b, and IRAK1c. Auto-phosphorylation plays a role in IRAK-1 activation and mediates proteasome-mediated degradation leading to IRAK1 protein loss. Meanwhile, IRAK1b lacks kinase activity and is resistant to proteasome-mediated degradation. Additionally, IRAK1c has a truncated sequence and is therefore mutated at the C-terminus of its kinase domain and acts as a negative regulator of the TLR and IL-1R signaling pathways.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human IRAK1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunocytochemistry (ICC), we recommend using 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation.

This product is not recommended for use in immunohistochemistry (IHC).

Additional Product Notes

This antibody has been tested in knockout/knockdown models for Western Blotting (WB).

RRID
AB_3097576 (BioLegend Cat. No. 632051)
AB_3097576 (BioLegend Cat. No. 632052)

Antigen Details

Structure
IRAK1 protein is a 712 amino acid protein with a predicted molecular weight of 77 kD
Distribution

Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Function
Serine/threonine protein kinase
Interaction
TRAF6, PELI1, IRAK4, MYD88, TOLLIP, IL1RL1, IKBKG/NEMO, IRAK4, PELI3, INAVA and NFATC4
Cell Type
Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Dinarello CA. 1996. Blood. 87:2095-147.
  2. Takaesu G, et al. 2001. Mol Cell Biol. 21: 2475-84.
  3. Janssens S & Beyaert R. 2003. Mol Cell. 11:293-302.
  4. Jain A, et al. 2014. Front Immunol. 5:553.
  5. Gottipati S, et al. 2008. Cell Signal. 20:269-76.
Gene ID
3654 View all products for this Gene ID
UniProt
View information about IRAK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/31/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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