Purified anti-c-Myc Antibody

Pricing & Availability
Clone
9E10 (See other available formats)
Regulatory Status
RUO
Other Names
Oncogene Myc, Myc proto-oncogene protein, proto-oncogene c-Myc
Isotype
Mouse IgG1, κ
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Product Citations
publications
a_9E10_PURE_c-Myc_Antibody_WB_062618
Total cell lysates (15 µg protein) from 293E cells transfected with Multi-tag (lane 1) or non-transfected cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL purified anti-c-Myc antibody (1:500 dilution), clone 9E10 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-Myc antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower blot). Lane M: Molecular weight ladder.
  • a_9E10_PURE_c-Myc_Antibody_WB_062618
    Total cell lysates (15 µg protein) from 293E cells transfected with Multi-tag (lane 1) or non-transfected cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL purified anti-c-Myc antibody (1:500 dilution), clone 9E10 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-Myc antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower blot). Lane M: Molecular weight ladder.
  • b_9E10_PURE_c-Myc_Antibody_WB_2_081821
    Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of recombinant Posi-tag epitope tag protein (Cat. No. 931301) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-c-Myc antibody (clone 9E10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • c_9E10_PURE_c-Myc_Antibody_IP_090721.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells mock-transfected (-) or transfected (+) with multi-tag plasmid containing c-Myc epitope sequence were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400101) or purified anti-c-Myc antibody (clone 9E10). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-c-Myc antibody (clone 9E11) at 1.0 µg/mL (1:1000 dilution). Lane M: Molecular weight marker.
  • d_9E10_PURE_c-Myc_Antibody_ICC_090721.png
    ICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 goat anti-mouse IgG antibody (green) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 100 µm
  • e_9E10_PURE_c-Myc_Antibody_IHC-P_090721.png
    IHC staining of anti-c-Myc antibody (clone 9E10) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using 0.1M Tris-buffered saline w/ Tween 20 (Cat. No. 925501), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody for one hour at room temperature. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 100 µm
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626801 25 µg £58.00
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626802 100 µg £127.00
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Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, IHC-P - Verified
Purification - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 1.0 - 2.5 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
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  3. Munro S, Pelham HR. 1987. Cell. 48:899.
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Product Citations
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  11. Candiracci J, et al. 2019. Sci Adv. 5:eaav0184. PubMed
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  14. Kodo K, et al. 2017. Sci Rep. 4.99375. PubMed
  15. Cohen–Khait R, et al. 2017. Structure. 25:1867. PubMed
  16. Quach C, et al. 2019. Nat Commun. 4.361805556. PubMed
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  18. Chen PH, et al. 2020. JCI Insight. 5:e127751. PubMed
  19. Stern LA, et al. 2019. ACS Comb Sci. 21:207. PubMed
  20. Umlauf BJ, et al. 2019. Sci Adv. 5:eaau4245. PubMed
  21. Torii T, et al. 2020. The Journal of Cell Biology. 219(2):e201907048. PubMed
  22. Kim JH, et al. 2020. Nucleic Acids Res. 48:10753. PubMed
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  24. Thibodeau M 2012. J Immunol. 188:4959. PubMed
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  26. Peters B, et al. 2012. Microbiology. 158:2975. PubMed
  27. Robu M, et al. 2013. Proc Natl Acad Sci U S A. 110:1658. PubMed
  28. Eilert E, et al. 2013. J Biotechnol. 167:85. PubMed
  29. Bi J, et al. 2013. Am J Physiol Renal Physiol. 305:532. PubMed
  30. Poe J, et al. 2013. J Exp Med. 511:57. PubMed
  31. Shkoporov A, et al. 2015. FEMS Microbiol Lett. 362:83. PubMed
  32. Rai R, et al. 2015. Genetics. 201: 989 - 1016. PubMed
  33. Muthusamy B, et al. 2015. PLoS Biol. 13(12): e1002325. PubMed
  34. Kennedy J, et al. 2015. PLoS One. 10: 0145466. PubMed
  35. Wang R, et al. 2016. Nat Commun. 7:10269. PubMed
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  37. García R, et al. 2016. J Cell Sci. 129: 1649-1660. PubMed
  38. Dowd G, et al. 2016. Infect Immun . 84: 1826 - 1841. PubMed
  39. Syed S, et al. 2016. PLoS Genet. 12:e1006451. PubMed
  40. GÉnier S, et al. 2016. Mol Biol Cell. 27(24):3800-3812. PubMed
  41. Bloch N, et al. 2017. J Biol Chem. 292(3):1068-1080. PubMed
  42. Xu W, et al. 2020. Immunohorizons. 0.631944444. PubMed
  43. Alme EB, et al. 2020. PLoS Genet. 16:e1008840. PubMed
  44. Sansó M, et al. 2020. Nucleic Acids Res. 48:7154. PubMed
  45. Takakura M, et al. 2021. Nat Commun. 0.936111111. PubMed
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RRID
AB_2235686 (BioLegend Cat. No. 626801)
AB_2148451 (BioLegend Cat. No. 626802)

Antigen Details

Structure
Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
Distribution

Nuclear protein

Function
Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
Interaction
MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
Modification
Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.

Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 09/07/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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