- 9E10 (See other available formats)
- Other Names
- Oncogene Myc, Myc proto-oncogene protein
- Mouse IgG1, κ
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- Product Citations
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The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. c-myc is part of a heterodimeric complex with MAX that acts as a potent transcriptional activator. c-myc is modified by glycosylation and phosphorylation and has been shown to interact with a number of proteins including SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, and Sp1. c-myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that the observed protein size is approximately 41 kD. The 9E10 monoclonal antibody recognizes human myc and the 10 amino acid epitope tag of human c-myc. The 9E10 antibody has been shown to be useful in a number of applications including Western blotting, direct ELISA, flow cytometry, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), and immunoaffinity purification of proteins expressing the human c-myc tag.Product Details
- Human and fusion protein (EQKLISEEDL tag epitope) in all species
- Antibody Type
- Host Species
- Amino acids 408-439, C-terminal region of human c-myc
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry5 of formalin-fixed, paraffin-embedded tissue sections, ELISA1, immunofluorescence microscopy3,4, immunoprecipitation4, and immunoaffinity of c-myc-tagged fusion proteins6.
(PubMed link indicates BioLegend citation)
- Schouten A, et al. 2002. J. Biol. Chem. 277:19339. (ELISA)
- Maher SE, et al. 1998. Transplantation 66:1094.
- Raftopoulou M, et al. 2004. Science 303:1179. (IF)
- Fan H, et al. 1998. Biochem. Cell. Biol. 76:125. (IF, IP)
- Korkolopoulou P, et al. 1994. Leuk Lymphoma 13:151. (IHC)
- Hillman MC, et al. 2001. Protein Expr. Purif. 23:359.
- Kondo, S., et al. 2011. J. Virol. 85:11255. PubMed
- Decarpentrie F, et al. 2012. Hum Mol Genet. PubMed
- Bourgeois-Daigneault MC, et al. 2012. J Immunol. 188:4959. PubMed
- Peters BM, et al. 2012. Microbiology. 158:2975. PubMed
- Robu M, et al. 2013. PNAS. 110:1658. PubMed
- Eilert E, et al. 2013. J Biotechnol. 167:85. PubMed
- Bi J, et al. 2013. Am J Physiol Renal Physiol. 305:532. PubMed
- Poe JC, et al. 2013. J exp Med. 511:57. PubMed
- Shkoporov AN, et al. 2015. FEMS Microbial Lett. 362:83. PubMed
AB_2783361 (BioLegend Cat. No. 626804)
AB_2572009 (BioLegend Cat. No. 626803)
- Highly conserved protein structure Drosophila to vertebrates. Contains HLH domain, coiled coil region, and leucine zipper domain, molecular weight approximately 62 kD
Ubiquitously expressed nuclear protein
- Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
- MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
- Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
- Antigen References
1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.
- Gene ID
- 4609 View all products for this Gene ID
- View information about c-Myc on UniProt.org
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