- 9E10 (See other available formats)
- Regulatory Status
- Other Names
- Myc proto-oncogene protein, proto-oncogene c-Myc, transcription factor p64, class E basic helix-loop-helix protein 39, avian myelocytomatosis viral oncogene homolog, myc-related translation/localization
- Mouse IgG1, κ
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- Product Citations
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The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.Product Details
- Antibody Type
- Host Species
- Amino acids 408-439, C-terminal region of human c-myc
- 50% anti-c-Myc-tag (9E10) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3.
- Affinity purified.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Purification of c-Myc tagged fusion protein from bacteria lysates - Quality tested
IP - Reported in the literature, not verified in house
- Recommended Usage
Resin binding capacity is greater than 0.5 mg/mL.
- Application Notes
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
(PubMed link indicates BioLegend citation)
- Rao H, et al. 2001. Nature. 410:955.
- Peters CS, et al. 2001. J Biol Chem. 276:13718.
- Munro S, Pelham HR. 1987. Cell. 48:899.
- Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
- Product Citations
- Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
- Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
- MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
- Cell Targets
- N-terminal, C-terminal tagged and internal fusion protein
- Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
- Antigen References
1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.
- Gene ID
- 4609 View all products for this Gene ID
- View information about c-myc on UniProt.org
|Purified anti-c-Myc||9E10||WB, ICC, IP, IHC-P, Purification|
|Anti-c-Myc Tag (9E10) Affinity Gel||9E10||Purification, IP|
|Biotin anti-c-Myc||9E10||WB, ICC|
|Direct-Blot™ HRP anti-c-Myc||9E10||WB|
|Alexa Fluor® 594 anti-c-Myc||9E10||ICC|
|Ultra-LEAF™ Purified anti-c-Myc||9E10||WB, ELISA, ICC, IP, IHC-P, Purification|
|Alexa Fluor® 647 anti-c-Myc||9E10||ICC|
|Alexa Fluor® 488 anti-c-Myc Antibody||9E10||ICC|
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