- Clone
- A22009C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
631251 | 25 µg | £124 | ||||
631252 | 100 µg | £312 |
The proto-oncogene c-Fos is the human homolog of the retroviral v-Fos oncogene, and is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the Activator Protein-1 complex (AP-1). This complex is involved in critical cellular processes such as inflammation, cell proliferation, differentiation, survival, hypoxia, bone development and angiogenesis which suggests c-Fos is an important therapeutic target for cancer, vascular remodeling, acute inflammation and autoimmune diseases. In addition, increases in c-Fos expression and activation are associated with neuronal activity induced by psychoactive drugs. Expression of c-Fos is rapid and transitory, and phosphorylation at residues Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human c-Fos phosphorylated at Ser32.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.012 - 0.1 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For Intracellular Flow Cytometry (ICFC), we recommend fixation/permeabilization with the True-Phos™ Perm Buffer (Cat. No. 425401). This antibody also works with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) with a small reduction on the Mean Fluorescence Intensity (MFI).
For immunocytochemistry (ICC), fixation/ permeabilization with 2% PFA/ice-cold methanol or 2% PFA/0.5% Triton X-100 is recommended - RRID
-
AB_3097545 (BioLegend Cat. No. 631251)
AB_3097545 (BioLegend Cat. No. 631252)
Antigen Details
- Structure
- c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins.
- Distribution
-
Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation/phosphorylation the protein is translocated to the nucleus.
- Function
- c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus.
- Interaction
- c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor.
- Ligand/Receptor
- As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors
- Molecular Family
- Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors
- Antigen References
-
- Sasaki T, et al. 2006. Mol Cell. 24:63-75.
- Huang K, et al. 2014. Cell Signal. 26:629-38.
- Qu X, et al. 2019. Cancer Sci. 110:3183-3196.
- Guo JC, et al. 2015. PLoS One. 10:e0120332.
- Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818.
- Gene ID
- 2353 View all products for this Gene ID
- UniProt
- View information about c-Fos Phospho (Ser32) on UniProt.org
Related FAQs
Other Formats
View All c-Fos Phospho Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-c-Fos Phospho (Ser32) | A22009C | WB,ICC,ICFC |
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