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PerCP/Cyanine5.5 anti-human CD57 Antibody

Pricing & Availability
Clone
HNK-1 (See other available formats)
Regulatory Status
RUO
Other Names
HNK-1, NK-1, Leu-7
Isotype
Mouse IgM, κ
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Product Citations
publications
HNK-1_PerCPCy55_CD57_Antibody_101618
Human peripheral blood lymphocytes were stained with CD8 APC and CD57 (clone HNK-1) PerCP/Cyanine5.5 (left), or mouse IgM, ? PerCP/Cyanine5.5 isotype control (right).
  • HNK-1_PerCPCy55_CD57_Antibody_101618
    Human peripheral blood lymphocytes were stained with CD8 APC and CD57 (clone HNK-1) PerCP/Cyanine5.5 (left), or mouse IgM, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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359621 25 tests £121
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359622 100 tests £262
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Description

CD57, also known as HNK-1, NK-1, and Leu-7 is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, and is also expressed on neural cells and striated muscle. CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets. While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Membrane extract of human lymphoblastoid cell line HSB-2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications for the relevant formats include: Western blotting1.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Yoshihara Y, et al. 1991. J. Cell Biol. 115:731. (WB)
  2. Abo T, et al. 1981. J. Immunol. 127:1024.
  3. Abo T, et al. 1982. J. Immunol. 129:1752.
  4. Abo T, et al. 1982. J. Immunol. 129:1758.
Product Citations
  1. Rizzo MD, et al. 2019. J Pharmacol Exp Ther. 371:191. PubMed
  2. Carre C, et al. 2021. iScience. 24:102970. PubMed
  3. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  4. Kabanova A, et al. 2016. Cell Rep. S2211-1247: 30219-4. PubMed
  5. Levy Y, et al. 2021. iScience. 24:102711. PubMed
  6. Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
  7. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  8. Fernandez IZ, et al. 2019. J Exp Med. 216:1255. PubMed
RRID
AB_2565929 (BioLegend Cat. No. 359621)
AB_2565930 (BioLegend Cat. No. 359622)

Antigen Details

Structure
Oligosaccharide antigenic determinant present on a variety of proteins, lipids, and chrondroitin sulfate proteoglycans, 100-115 kD. The antigen is conserved across species.
Distribution

CD57 antigen is expressed on a subset of peripheral blood lymphocytes including a subset of NK cells and a subset of CD8+ T cells. Also expressed on neural cells and striated muscle.

Function
Unknown function, may be involved in cell-matrix interactions.
Ligand/Receptor
Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M, et al. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.

Gene ID
27087 View all products for this Gene ID
UniProt
View information about CD57 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 10/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Alexa Fluor® 647 anti-human CD57

  • Biotin anti-human CD57

  • PE/Dazzle™ 594 anti-human CD57

  • PerCP/Cyanine5.5 anti-human CD57

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  • Alexa Fluor® 594 anti-human CD57
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