PerCP/Cyanine5.5 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_PerCPCy55_CD19_Antibody_101618
Human peripheral blood lymphocytes were stained with CD19 (clone HIB19) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • HIB19_PerCPCy55_CD19_Antibody_101618
    Human peripheral blood lymphocytes were stained with CD19 (clone HIB19) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
302229 25 tests £94
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302230 100 tests £197
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. He WT, et al. 2022. Sci Transl Med. 14:eabl9605. PubMed
  2. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  3. Abarca-Zabalía J, et al. 2023. Front Immunol. 14:1127352. PubMed
  4. Zhou P, et al. 2023. Immunity. 56:669. PubMed
  5. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  6. Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed
  7. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  8. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  9. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  10. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  11. Munteanu AN, et al. 2019. Exp Ther Med. 18:1693. PubMed
  12. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  13. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  14. Sung BY, et al. 2022. J Clin Invest. 132:. PubMed
  15. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  16. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  17. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  18. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  19. Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed
  20. Rice TF, et al. 2021. EBioMedicine. 72:103612. PubMed
  21. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  22. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  23. Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
  24. He WT, et al. 2022. Nat Immunol. 23:960. PubMed
  25. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  26. Zumaquero E, et al. 2019. Elife. 8. PubMed
  27. Garcia-Carmona Y, et al. 2015. Blood. 125:1749. PubMed
  28. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  29. Graham C, et al. 2021. Immunity. 54(6):1276-1289.e6. PubMed
  30. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  31. Seow J, et al. 2022. Cell Rep. 39:110757. PubMed
  32. Lev A, et al. 2021. J Exp Med. 218:. PubMed
  33. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  34. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  35. Adhikaree J, et al. 2019. Oncoimmunology. 8:1593803. PubMed
  36. Calzoni E, et al. 2019. J Allergy Clin Immunol. 143:2317. PubMed
  37. Abolhalaj M, et al. 2022. Cancer Med. 11:3023. PubMed
  38. Fucà G, et al. 2021. J Immunother Cancer. 9:. PubMed
  39. Inui M, et al. 2015. Int Immunol. 27: 345 - 355. PubMed
  40. Fraser H, et al. 2015. J Immunol. 195: 4841 - 4852. PubMed
  41. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  42. Sweeney CL, et al. 2021. Gene Ther. 28:373. PubMed
  43. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  44. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  45. Duncan CJA, et al. 2022. J Exp Med. 219:. PubMed
  46. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  47. Sullivan KD et al. 2016. eLife. 5 pii: e16220. PubMed
  48. Lubaki N, et al. 2016. PLoS Pathog. 12:e1006031. PubMed
  49. Lepore M, et al. 2014. J Exp Med. 211:1363. PubMed
  50. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_2275547 (BioLegend Cat. No. 302229)
AB_2073119 (BioLegend Cat. No. 302230)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD19 HIB19 FC
Biotin anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Purified anti-human CD19 HIB19 FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
Alexa Fluor® 488 anti-human CD19 HIB19 FC,ICC
Alexa Fluor® 647 anti-human CD19 HIB19 FC,IHC-F
Pacific Blue™ anti-human CD19 HIB19 FC
Alexa Fluor® 700 anti-human CD19 HIB19 FC
PerCP anti-human CD19 HIB19 FC
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Brilliant Violet 421™ anti-human CD19 HIB19 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19 HIB19 FC
Brilliant Violet 650™ anti-human CD19 HIB19 FC
Brilliant Violet 785™ anti-human CD19 HIB19 FC
Brilliant Violet 510™ anti-human CD19 HIB19 FC
Brilliant Violet 605™ anti-human CD19 HIB19 FC
Brilliant Violet 711™ anti-human CD19 HIB19 FC
Purified anti-human CD19 (Maxpar® Ready) HIB19 FC,CyTOF®
Alexa Fluor® 594 anti-human CD19 HIB19 ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
Pacific Blue™ anti-human CD19 HIB19 FC
APC anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
TotalSeq™-A0050 anti-human CD19 HIB19 PG
Brilliant Violet 750™ anti-human CD19 HIB19 FC
TotalSeq™-B0050 anti-human CD19 HIB19 PG
TotalSeq™-C0050 anti-human CD19 HIB19 PG
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Spark NIR™ 685 anti-human CD19 HIB19 FC
Ultra-LEAF™ Purified anti-human CD19 HIB19 FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19 HIB19 FC
PE/Fire™ 640 anti-human CD19 HIB19 FC
PE/Fire™ 700 anti-human CD19 HIB19 FC
TotalSeq™-D0050 anti-human CD19 HIB19 PG
Spark YG™ 593 anti-human CD19 HIB19 FC
GMP Pacific Blue™ anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
GMP PE anti-human CD19 HIB19 FC
GMP APC anti-human CD19 HIB19 FC
KIRAVIA Blue 520™ anti-human CD19 HIB19 FC
GMP PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
GMP PE/Cyanine7 anti-human CD19 HIB19 FC
Spark Violet™ 500 anti-human CD19 HIB19 FC
PE/Fire™ 810 anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
Spark UV™ 387 anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Alexa Fluor® 660 anti-human CD19 HIB19 FC
PerCP/Fire™ 806 anti-human CD19 HIB19 FC
PerCP/Fire™ 780 anti-human CD19 HIB19 FC
Spark PLUS UV™ 395 anti-human CD19 HIB19 FC
Spark Red™ 718 anti-human CD19 HIB19 FC
Go To Top Version: 3    Revision Date: 10/16/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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