- J252D4 (See other available formats)
- Regulatory Status
- Other Names
- BLR1, MDR15
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human CXCR5-transfected cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Product Citations
AB_2561818 (BioLegend Cat. No. 356909)
AB_2561819 (BioLegend Cat. No. 356910)
- G-protein coupled receptor, 7 transmembrane regions, 42 kD
Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
- Mediates cell migration to the B cell follicles in the secondary lymphoid organs
- Cell Type
- B cells, Tfh
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.
- Gene ID
- 643 View all products for this Gene ID
- View information about CD185 on UniProt.org
Related Pages & Pathways
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other FormatsView All CD185 Reagents Request Custom Conjugation
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-human CD185 (CXCR5)
APC anti-human CD185 (CXCR5)
Purified anti-human CD185 (CXCR5)
Alexa Fluor® 647 anti-human CD185 (CXCR5)
PerCP/Cyanine5.5 anti-human CD185 (CXCR5)
Alexa Fluor® 488 anti-human CD185 (CXCR5)
FITC anti-human CD185 (CXCR5)
Alexa Fluor® 700 anti-human CD185 (CXCR5)
Pacific Blue™ anti-human CD185 (CXCR5)
Brilliant Violet 421™ anti-human CD185 (CXCR5)
PE/Cyanine7 anti-human CD185 (CXCR5)
APC/Cyanine7 anti-human CD185 (CXCR5)
PE/Dazzle™ 594 anti-human CD185 (CXCR5)
Brilliant Violet 605™ anti-human CD185 (CXCR5)
Biotin anti-human CD185 (CXCR5)
Brilliant Violet 711™ anti-human CD185 (CXCR5)
Brilliant Violet 785™ anti-human CD185 (CXCR5)
TotalSeq™-A0144 anti-human CD185 (CXCR5)
TotalSeq™-C0144 anti-human CD185 (CXCR5)
Brilliant Violet 750™ anti-human CD185 (CXCR5)
TotalSeq™-B0144 anti-human CD185 (CXCR5)
APC/Fire™ 750 anti-human CD185 (CXCR5)
Spark NIR™ 685 anti-human CD185 (CXCR5)
TotalSeq™-D0144 anti-human CD185 (CXCR5)
PE/Cyanine5 anti-human CD185 (CXCR5)
PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody
APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody