PE anti-human CD185 (CXCR5) Antibody

Pricing & Availability
J252D4 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were stained with CD19 FITC and CD185 (CXCR5, clone J252D4) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • J252D4_PE_CXCR5_Antibody_1_020713
    Human peripheral blood lymphocytes were stained with CD19 FITC and CD185 (CXCR5, clone J252D4) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • J252D4_PE_CXCR5_Antibody_2_020713
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Cat # Size Price Quantity Check Availability Save
356903 25 tests £100
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356904 100 tests £258
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CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human CXCR5-transfected cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Carre C, et al. 2021. iScience. 24:102970. PubMed
  2. Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed
  3. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  4. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  5. Kaneko N, et al. 2020. Cell. 183(1):143-157.e13. PubMed
  6. Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
  7. Seo IH, et al. 2021. Immune Netw. 21:e17. PubMed
  8. Woodruff M, et al. 2020. medRxiv. . PubMed
  9. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  10. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  11. Schultheiß C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed
  12. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  13. Li G, et al. 2021. Mol Ther Oncolytics. 22:507. PubMed
AB_2561812 (BioLegend Cat. No. 356903)
AB_2561813 (BioLegend Cat. No. 356904)

Antigen Details

G-protein coupled receptor, 7 transmembrane regions, 42 kD

Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.

Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.

Gene ID
643 View all products for this Gene ID
View information about CD185 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 02/07/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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