PE/Fire™ 810 anti-human TIGIT (VSTM3) Antibody

Pricing & Availability
A15153G (See other available formats)
Regulatory Status
Other Names
T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
Human peripheral blood lymphocyte were stained with anti-human CD3 FITC and anti-human TIGIT (clone A15153G) PE/Fire™ 810 (left) or stained with anti-human CD3-FITC only (right).
  • A15153G_PE-Fire-810_TIGIT_Antibody_082422
    Human peripheral blood lymphocyte were stained with anti-human CD3 FITC and anti-human TIGIT (clone A15153G) PE/Fire™ 810 (left) or stained with anti-human CD3-FITC only (right).
Compare all formats See PE/Fire™ 810 spectral data
Cat # Size Price Quantity Check Availability Save
372745 25 tests £121
Check Availability

Need larger quantities of this item?
Request Bulk Quote

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant Human TIGIT.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.


* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking1.

Application References

(PubMed link indicates BioLegend citation)
  1. Stamm H, et al. 2018. Oncogene. Pubmed
AB_2924566 (BioLegend Cat. No. 372745)

Antigen Details

26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.

Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.

Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type
NK cells, T cells, Tfh, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.

Gene ID
201633 View all products for this Gene ID
View information about TIGIT on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/14/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human TIGIT (VSTM3)

  • APC/Fire™ 750 anti-human TIGIT (VSTM3)

  • APC anti-human TIGIT (VSTM3)

  • PE anti-human TIGIT (VSTM3)

  • Brilliant Violet 421™ anti-human TIGIT (VSTM3)

  • Brilliant Violet 605™ anti-human TIGIT (VSTM3)

  • PE/Dazzle™ 594 anti-human TIGIT (VSTM3)

  • PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)

  • PE/Cyanine7 anti-human TIGIT (VSTM3)

  • Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)

  • Biotin anti-human TIGIT (VSTM3)

  • Alexa Fluor® 647 anti-human TIGIT (VSTM3)

  • TotalSeq™-A0089 anti-human TIGIT (VSTM3)

  • TotalSeq™-B0089 anti-human TIGIT (VSTM3)

  • TotalSeq™-C0089 anti-human TIGIT (VSTM3)

  • KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)

  • APC/Cyanine7 anti-human TIGIT (VSTM3)

  • Brilliant Violet 510™ anti-human TIGIT (VSTM3)

  • Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody

  • TotalSeq™-D0089 anti-human TIGIT (VSTM3)

  • Brilliant Violet 711™ anti-human TIGIT (VSTM3)

  • PE/Fire™ 640 anti-human TIGIT (VSTM3)

  • PE/Fire™ 810 anti-human TIGIT (VSTM3)

  • PE/Cyanine5 anti-human TIGIT (VSTM3)

  • PerCP/Fire™ 806 anti-human TIGIT (VSTM3)


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account