APC anti-human TIGIT (VSTM3) Antibody

Pricing & Availability
Clone
A15153G (See other available formats)
Regulatory Status
RUO
Other Names
T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A15153G_APC_TIGIT_Antibody_1_102516
Human peripheral blood leukocytes were stained with CD3 FITC and TIGIT (clone A15153G) APC (top) or mouse IgG2a, κ APC isotype control (bottom). Data shown was gated on a lymphocyte population.
  • A15153G_APC_TIGIT_Antibody_1_102516
    Human peripheral blood leukocytes were stained with CD3 FITC and TIGIT (clone A15153G) APC (top) or mouse IgG2a, κ APC isotype control (bottom). Data shown was gated on a lymphocyte population.
  • A15153G_APC_TIGIT_Antibody_2_102516
See APC spectral data
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372705 25 tests £99.00
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372706 100 tests £236.00
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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human TIGIT.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking1.

Application References

(PubMed link indicates BioLegend citation)
  1. Stamm H, et al. 2018. Oncogene. Pubmed
Product Citations
  1. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  2. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  3. Neidleman J, et al. 2020. Elife. 9:00. PubMed
  4. Market M, et al. 2020. Front Immunol. 1.127083333. PubMed
RRID
AB_2632731 (BioLegend Cat. No. 372705)
AB_2632732 (BioLegend Cat. No. 372706)

Antigen Details

Structure
26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
Distribution

Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.

Function
Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
Ligand/Receptor
CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type
NK cells, T cells, Tfh, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.

Gene ID
201633 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/27/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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