PE/Dazzle™ 594 anti-human CD85d (ILT4) Antibody

Pricing & Availability
Clone
42D1 (See other available formats)
Other Names
CD85, ILT4, MIR10, Leukocyte Immunoglobulin-Like Receptor subfamily B member 2 (LILRB2), Leukocyte Immunoglobulin-like Receptor 2 (LIR-2)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
42D1_PEDazzle594_CD85d_Antibody_060418
Human peripheral blood monocytes were stained with CD85d (ILT4, clone 42D1) PE/Dazzle™ 594 (filled histogram) or Rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram) in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426103).
  • 42D1_PEDazzle594_CD85d_Antibody_060418
    Human peripheral blood monocytes were stained with CD85d (ILT4, clone 42D1) PE/Dazzle™ 594 (filled histogram) or Rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram) in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426103).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
338709 25 tests £82
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338710 100 tests £219
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Description

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). It is composed of both activating and inhibitory isoforms. The activating subset of ILTs is characterized by containing short cytoplasmic domains and positively charged arginine residues, while the inhibitory isoforms display long cytoplasmic tails containing ITIM motifs. CD85d is a 95kD inhibitory receptor, also known as ILT4, LIR2, or MIR10. ILT4 is expressed on the surface of monocytes/macrophages, and dendritic cells. ILT4 acts as an inhibitory receptor through recruitment of SHP-1 and SHP-2 protein tyrosine phosphatases. The ligands of ILT4 are HLA-A, -B and nonclassical MHC-I molecule HLA-G1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: negatively modulate myelomonocytic cells signaling. Enhance the binding of HLA-G tetramer to monocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Colonna M, et al. 1998. J. Immunol. 160:3096 
  2. Allan DS. 1999. J. Exp. Med. 189:1149
RRID
AB_2734343 (BioLegend Cat. No. 338709)
AB_2734344 (BioLegend Cat. No. 338710)

Antigen Details

Structure
ILT/LIR family, Ig superfamily, 95kD
Distribution

Monocytes/macrophages, dendritic cells

Ligand/Receptor
MHC class I molecules (HLA-A, -B) and nonclassic class I molecule HLA-G1
Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication 
  2. Shiroishi M, et al. 2003. Proc. Natl. Acad. Sci. 100:8856 
  3. Colonna M, et al. 1998. J. Immunol. 160:3096 
  4. Lichterfeld M, et al. 2007. J. Exp. Med. 204:2813
Gene ID
10288 View all products for this Gene ID
UniProt
View information about CD85d on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/04/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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