Loading...

PE/Cyanine7 anti-human CD85d (ILT4) Antibody

Pricing & Availability
Clone
42D1 (See other available formats)
Regulatory Status
RUO
Other Names
CD85, ILT4, MIR10, Leukocyte Immunoglobulin-Like Receptor subfamily B member 2 (LILRB2), Leukocyte Immunoglobulin-like Receptor 2 (LIR-2)
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
42D1_PECy7_CD85d_Antibody_080918
Human peripheral blood monocytes were stained with CD85d (ILT4, clone 42D1) PE/Cyanine7 (filled histogram) or Rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • 42D1_PECy7_CD85d_Antibody_080918
    Human peripheral blood monocytes were stained with CD85d (ILT4, clone 42D1) PE/Cyanine7 (filled histogram) or Rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
338711 25 tests £105
Check Availability


Need larger quantities of this item?
Request Bulk Quote
338712 100 tests £249
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). It is composed of both activating and inhibitory isoforms. The activating subset of ILTs is characterized by containing short cytoplasmic domains and positively charged arginine residues, while the inhibitory isoforms display long cytoplasmic tails containing ITIM motifs. CD85d is a 95kD inhibitory receptor, also known as ILT4, LIR2, or MIR10. ILT4 is expressed on the surface of monocytes/macrophages, and dendritic cells. ILT4 acts as an inhibitory receptor through recruitment of SHP-1 and SHP-2 protein tyrosine phosphatases. The ligands of ILT4 are HLA-A, -B and nonclassical MHC-I molecule HLA-G1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: negatively modulate myelomonocytic cells signaling. Enhance the binding of HLA-G tetramer to monocytes.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Colonna M, et al. 1998. J. Immunol. 160:3096 
  2. Allan DS. 1999. J. Exp. Med. 189:1149
RRID
AB_2750150 (BioLegend Cat. No. 338711)
AB_2750151 (BioLegend Cat. No. 338712)

Antigen Details

Structure
ILT/LIR family, Ig superfamily, 95kD
Distribution

Monocytes/macrophages, dendritic cells

Ligand/Receptor
MHC class I molecules (HLA-A, -B) and nonclassic class I molecule HLA-G1
Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication 
  2. Shiroishi M, et al. 2003. Proc. Natl. Acad. Sci. 100:8856 
  3. Colonna M, et al. 1998. J. Immunol. 160:3096 
  4. Lichterfeld M, et al. 2007. J. Exp. Med. 204:2813
Gene ID
10288 View all products for this Gene ID
UniProt
View information about CD85d on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/09/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD85d (ILT4)

  • APC anti-human CD85d (ILT4)

  • PE/Dazzle™ 594 anti-human CD85d (ILT4)

  • PE/Cyanine7 anti-human CD85d (ILT4)

  • Ultra-LEAF™ Purified anti-human CD85d (ILT4)

  • TotalSeq™-B1249 anti-human CD85d (ILT4)

  • TotalSeq™-D1249 anti-human CD85d (ILT4)

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account