PE/Cyanine7 anti-human CD336 (NKp44) Antibody

Pricing & Availability
Clone
P44-8 (See other available formats)
Regulatory Status
RUO
Other Names
NKp44, NCR2, Activating NK receptor NKp44, natural cytotoxicity triggering receptor 2. lymphocyte antigen 95 homolog
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P44dash8_PECy7_CD336_Antibody_1_041416
Human peripheral blood lymphocytes were stimulated with recombinant human IL-2 for 7 days and then stained with CD56 APC and CD336 (clone P44-8) PE/Cyanine7 (top) or mouse IgG1, κ PE/Cyanine7 isotype control (bottom).
  • P44dash8_PECy7_CD336_Antibody_1_041416
    Human peripheral blood lymphocytes were stimulated with recombinant human IL-2 for 7 days and then stained with CD56 APC and CD336 (clone P44-8) PE/Cyanine7 (top) or mouse IgG1, κ PE/Cyanine7 isotype control (bottom).
  • P44dash8_PECy7_CD336_Antibody_2_041416
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
325115 25 tests £92.00
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325116 100 tests £222.00
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Description

CD336 is also known as activating NK receptor NKp44 (NKp44), natural cytotoxicity triggering receptor 2, and lymphocyte antigen 95 homolog. It is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp44 has an apparent molecular weight of 44 kD and three isoforms are produced by alternative splicing. NKp44 is expressed on IL-2 activated NK cells and a subset of γ/δ T cells. NKp44 enhances NK cell mediated cytolysis of virus infected cells and tumor cells. NKp44 has been shown to associate with the intracellular adaptor DAP12.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant human NKp44
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The p44-8 antibody against human NKp44 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution. Additional reported applications (for the relevant formats) include: stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution1,2. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 325104).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Sommaggio R, et al. 2012. J. Immunol. 188:2075. (Block)
  2. Hoechst B, et al. 2009. Hepatology. 50:799. (Block) PubMed
  3. Correia DV, et al. 2011. Blood. 118:992. (FC) PubMed
Product Citations
  1. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  2. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  3. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
RRID
AB_2616753 (BioLegend Cat. No. 325115)
AB_2616754 (BioLegend Cat. No. 325116)

Antigen Details

Structure
Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Three isoforms produced by alternative splicing.
Distribution

IL-2 activated NK cells and cell lines, some γ/δ T cells activated in vitro

Function
NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction
DAP12
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Cantoni C, et al. 1999. J. Exp. Med. 189:787.
2. Allcock RJN, et al. 2003. Eur. J. Immunol. 33:567.
3. Cantoni C, et al. 2003. Structure. 11:725.

Gene ID
9436 View all products for this Gene ID
UniProt
View information about CD336 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/15/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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