- S20013B (See other available formats)
- Regulatory Status
- Other Names
- T44, Tp44
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human CD28 transfected cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone S20013B antibody can completely block the binding of clone CD28.2 on target.
Clone S20013B can partially block Clone S20013F on target.
Clone S20013B can increase anti-CD3 induced human peripheral T-cell proliferation.
Clone S20013B is a superagonistic antibody raised towards human CD28, and can induce activation, proliferation of human peripheral T cells without anti-CD3 stimulation.
AB_2936698 (BioLegend Cat. No. 377811)
AB_2936698 (BioLegend Cat. No. 377812)
- Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
- T cell costimulation
- CD80, CD86
- Cell Type
- Lymphocytes, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
- Biology Area
- Adaptive Immunity, Cell Proliferation and Viability, Costimulatory Molecules, Immuno-Oncology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
- Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
- June CH, et al. 1994. Immunol Today. 15:321-31.
- Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212.
- Gene ID
- 940 View all products for this Gene ID
- View information about CD28 on UniProt.org
|Ultra-LEAF™ Purified anti-human CD28 (Superagonistic)||S20013B||FC,Activ,Costim|
|APC anti-human CD28||S20013B||FC|
|FITC anti-human CD28||S20013B||FC|
|PE anti-human CD28||S20013B||FC|
|PE/Cyanine7 anti-human CD28||S20013B||FC|
|PerCP/Cyanine5.5 anti-human CD28||S20013B||FC|
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