PE/Cyanine7 anti-human CD162 Antibody

Pricing & Availability
Clone
KPL-1 (See other available formats)
Other Names
PSGL-1, p-selectin glycoprotein ligand-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
KPL-1_PECyanine7_CD162_Antibody_050119.png
Human peripheral blood lymphocytes were stained with CD3 APC and CD162 (clone KPL-1) PE/Cyanine7 (left) or Mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • KPL-1_PECyanine7_CD162_Antibody_050119.png
    Human peripheral blood lymphocytes were stained with CD3 APC and CD162 (clone KPL-1) PE/Cyanine7 (left) or Mouse IgG1, κ PE/Cyanine7 isotype control (right).
Cat # Size Price Quantity Avail. Save
328815 25 tests £87
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328816 100 tests £185
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Description

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kD, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PSGL-1 transfected murine 300.19 pre B-cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11. Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections1, blocks the recognition of PSGL-1 with P- and L-selectin1.

Application References

(PubMed link indicates BioLegend citation)
  1. Snapp KR, et al. 1998. Blood 91:154.
  2. Snapp KR, et al. 1998. J. Cell Biol. 142:263.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  4. Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
  5. Cheng Q, et al. 2012. Lupus. 21:632. PubMed.

Antigen Details

Distribution

neutrophils, monocytes, and most lymphocytes

Function
mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
Ligand/Receptor
CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
Cell Type
Neutrophils, Monocytes, Lymphocytes
Biology Area
Immunology, Cell Adhesion
Molecular Family
CD Molecules, Adhesion Molecules
Antigen References

1. Snapp KR, et al. 1998. Blood 91:154.

Gene ID
6404 View all products for this Gene ID
UniProt
View information about CD162 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/01/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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