PE anti-human IFN-γ Antibody

Pricing & Availability
Clone
W19227C (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W19227C_PE_IFN-gamma_Antibody_080422
PMA+Ionomycin (4 hours) stimulated human PBMCs were surface stained with anti-human CD3 APC then fixed, permeabilized, and intracellularly stained with anti-human IFN-γ (clone W19227C) PE (left), or rat IgG2b, λ PE isotype control (right).
  • W19227C_PE_IFN-gamma_Antibody_080422
    PMA+Ionomycin (4 hours) stimulated human PBMCs were surface stained with anti-human CD3 APC then fixed, permeabilized, and intracellularly stained with anti-human IFN-γ (clone W19227C) PE (left), or rat IgG2b, λ PE isotype control (right).
See PE spectral data
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383403 25 tests £69
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383404 100 tests £168
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Description

Human interferon-gamma (IFN-γ, type II interferon) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells in response to antigens and other cytokines. Originally characterized based on anti-viral activities. IFN-γ also exerts tumor anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II, and macrophage activation. This human IFN-γ antibody does not show cross-reactivity with mouse.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2924584 (BioLegend Cat. No. 383403)
AB_2924584 (BioLegend Cat. No. 383404)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Distribution

CD8+ and CD4+ T cells, NK cells

Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH 2
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
  2. De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
  3. Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611.
  4. Gray P, et al. 1987. Lymphokines. 13:151.
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/04/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • PE anti-human IFN-γ

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