|439007||1 Pre-coated Plate||£202|
Interferon-γ (IFN-γ) is the sole member of the type II interferon category. It is a potent multifunctional cytokine involved in both the innate and adaptive (especially cell-mediated) arms of immunity. It is secreted largely by activated lymphocytes, in particular, CD4+ Th1 cells, CD8+ cytotoxic cells, and NK cells.
Human IFN-γ is expressed as a pre-cursor protein with a signaling peptide which is cleaved to produce the mature protein of ~17 kD. Its active form is a homodimer, linked by anti-parallel non-covalent interactions between the six α-helices of each monomer. The receptor for IFN-γ is also a homodimer, with α & β subunits (IFNGR1 and IFNGR2, respectively). IFN-γ binds to IFNGR1 very specifically, and the subsequent interaction of IFNGR1 with IFNGR2 activates the Jak/Stat pathway for signal transduction and, ultimately, transcription of effector genes. Rat IFN-γ has 87% amino acid identity with mouse IFN-γ and 39% identity with the human protein. As such, rat IFN-γ can activate mouse cells but not human cells.
Originally characterized based on anti-viral properties, IFN-γ also exerts anti-microbial, anti-tumor, immunomodulatory, and pro-inflammatory activities. It is unique among the interferons for its ability to coordinate the transition from innate to adaptive immunity. Among its more significant roles, IFN-γ stimulates macrophage effector functions. It is also the major player in inducing Th1 cell differentiation and provides a positive feedback loop in response to IL-12 stimulation in defining Th1 cells.
BioLegend's LEGEND MAX™ Rat IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a rat IFN-γ-specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend's LEGEND MAX™ Rat IFN-γ ELISA Kit is specifically designed for the accurate quantitation of rat IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Kit Contents
- Anti-Rat IFN-γ Pre-coated 96-well Strip Microplate
- Rat IFN-γ Dectection Antibody
- Rat IFN-γ Standard
- Avidin-HRP D
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- 3.2 pg/mL
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.