FITC anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
IM7
C57BL/6 splenocytes stained with IM7 FITC
  • IM7
    C57BL/6 splenocytes stained with IM7 FITC
  • IM7_FITC_060710
    Human peripheral blood lymphocytes stained with IM7 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
103021 25 tests £29
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103005 50 µg £33
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103006 500 µg £76
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103022 100 tests £77
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
test size: lot-specific; µg size: 0.5 mg/ml
Storage & Handling
The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang S, et al. 2019. Int J Oncol. 54:966. PubMed
  2. Yang L, et al. 2022. Front Oncol. 12:862250. PubMed
  3. Ye ZW, et al. 2022. Cell Mol Immunol. 19:588. PubMed
  4. Tan L, et al. 2022. Aging Dis. 13:1562. PubMed
  5. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  6. Israelow B, et al. 2020. bioRxiv. . PubMed
  7. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  8. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  9. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  10. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  11. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  12. Dong L, et al. 2021. Cancer Cell. . PubMed
  13. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  14. Wang D, et al. 2018. Immunity. 48:659. PubMed
  15. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  16. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  17. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  18. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  19. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  20. Pack AD, et al. 2021. Cell Reports. 36(8):109586. PubMed
  21. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  22. Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
  23. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  24. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  25. Diao L, et al. 2022. iScience. 25:105511. PubMed
  26. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  27. Kmieciak M, et al. 2011. Breast Cancer Res Treat. 126:385. PubMed
  28. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  29. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  30. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  31. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  32. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  33. Bagheri V et al. 2018. Journal of cellular physiology. 233(10):7036-7046 . PubMed
  34. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  35. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  36. Yang L, et al. 2021. JCI Insight. 6:. PubMed
  37. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  38. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  39. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  40. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  41. Murai T, et al. 2013. J Cell Sci. 126:3284. PubMed
  42. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  43. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  44. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  45. Zhang W, et al. 2021. Cell Prolif. 54:e13058. PubMed
  46. Wang Z, et al. 2022. Ann Transl Med. 10:544. PubMed
  47. Ebina-Shibuya R, et al. 2021. Elife. 10:. PubMed
  48. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  49. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  50. Daniel CJ, et al. 2022. Mol Cancer Res. 20:1151. PubMed
  51. Fujiwara H, et al. 2021. Nat Immunol. 22:1440. PubMed
  52. Amici SA, et al. 2021. Front Immunol. 12:695947. PubMed
  53. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  54. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  55. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  56. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  57. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  58. Park SG, et al. 2021. J Vet Sci. 22:e16. PubMed
  59. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  60. Xia H, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L926. PubMed
  61. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  62. Wan X, et al. 2018. Nature. 560:107. PubMed
  63. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  64. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  65. Wu X, et al. 2022. Cell Death Dis. 13:557. PubMed
  66. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  67. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  68. Sim S, et al. 2016. PLoS One. 11: 0152485. PubMed
  69. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  70. Zhang S, et al. 2021. Stem Cells Int. 6616240:2021. PubMed
  71. Ahmed MF, et al. 2019. Exp Ther Med. 17:4154. PubMed
  72. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  73. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  74. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  75. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  76. Liu ZZ, et al. 2021. Autophagy. 17:2766. PubMed
  77. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  78. Liu Z, et al. 2016. Sci Rep. 6:34416. PubMed
  79. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  80. Kamijo S, et al. 2013. J Immunol. 190:4489. PubMed
  81. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  82. Matossian MD, et al. 2019. BMC Cancer. 19:205. PubMed
  83. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  84. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  85. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  86. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  87. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  88. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  89. Geburek F, et al. 2017. Stem Cell Res Ther. 8:129. PubMed
  90. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  91. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  92. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  93. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  94. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  95. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  96. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  97. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  98. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  99. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  100. Witschen PM, et al. 2020. Cancers. 12(5):1325. PubMed
  101. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  102. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  103. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  104. Byrne K, et al. 2014. J Immunol. 192:1433. PubMed
  105. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  106. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  107. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  108. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  109. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  110. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  111. Shah D, et al. 2018. Clin Cancer Res. 24:4566. PubMed
  112. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  113. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  114. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  115. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  116. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  117. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  118. Yang L, et al. 2022. Proteomics. 22:e2200018. PubMed
  119. Bi R, et al. 2020. Osteoarthritis Cartilage. 28:842. PubMed
  120. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  121. Zhao Y, et al. 2020. Br J Cancer. 1638:122. PubMed
  122. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  123. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  124. Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed
  125. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  126. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
RRID
AB_493684 (BioLegend Cat. No. 103021)
AB_312956 (BioLegend Cat. No. 103005)
AB_312957 (BioLegend Cat. No. 103006)
AB_493685 (BioLegend Cat. No. 103022)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC, ICC, IHC-P
FITC anti-mouse/human CD44 IM7 FC, ICC, IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC, IHC-F, CyTOF®, ELISA, ICC, IHC-P, IP, CMCD, Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC, ICC, IHC-P, SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC, ICC, SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC, SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC, ICC, IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC, CyTOF®, ELISA, IHC, IP, CMCD, Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC, CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC, FC, IHC-P, SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC, IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Go To Top Version: 2    Revision Date: 09/11/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse/human CD44

  • Biotin anti-mouse/human CD44

  • FITC anti-mouse/human CD44

  • PE/Cyanine5 anti-mouse/human CD44

  • Purified anti-mouse/human CD44

  • Brilliant Violet 605™ anti-mouse/human CD44

  • PE anti-mouse/human CD44

  • Alexa Fluor® 488 anti-mouse/human CD44

  • Alexa Fluor® 647 anti-mouse/human CD44

  • Pacific Blue™ anti-mouse/human CD44

  • Alexa Fluor® 700 anti-mouse/human CD44

  • PE/Cyanine7 anti-mouse/human CD44

  • APC/Cyanine7 anti-mouse/human CD44

  • PerCP/Cyanine5.5 anti-mouse/human CD44

  • PerCP anti-mouse/human CD44

  • Brilliant Violet 421™ anti-mouse/human CD44

  • Brilliant Violet 570™ anti-mouse/human CD44

  • Brilliant Violet 785™ anti-mouse/human CD44

  • Brilliant Violet 510™ anti-mouse/human CD44

  • Ultra-LEAF™ Purified anti-mouse/human CD44

  • Brilliant Violet 650™ anti-mouse/human CD44

  • Purified anti-mouse/human CD44 (Maxpar® Ready)

  • Alexa Fluor® 594 anti-mouse/human CD44

  • PE/Dazzle™ 594 anti-mouse/human CD44

  • Brilliant Violet 711™ anti-mouse/human CD44

  • APC/Fire™ 750 anti-mouse/human CD44

  • TotalSeq™-A0073 anti-mouse/human CD44

  • TotalSeq™-C0073 anti-mouse/human CD44

  • TotalSeq™-B0073 anti-mouse/human CD44

  • Spark YG™ 570 anti-mouse/human CD44

  • Spark YG™ 593 anti-mouse/human CD44

  • TotalSeq™-D0073 anti-mouse/human CD44

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