Brilliant Violet 650 anti-human CD3 Antibody anti-CD3

Pricing & Availability

Clone: UCHT1 (See other available formats)
Regulatory Status: RUO
Workshop: III 471
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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Product Citations: publications

UCHT1_BV650_CD3_Antibody_071216 — Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).

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300467

25 tests

£138

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300468

100 tests

£271

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections¹¹, immunoprecipitation¹, activation of T cells^2,3,5, Western blotting⁹, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References (PubMed link indicates BioLegend citation)

Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
Li J, et al. 2015. Cancer Res. 75:508. PubMed
Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Hasaart KAL, et al. 2020. Sci Rep. 10:12991. PubMed
Oh DY, et al. 2020. Cell. 181:1612. PubMed
Swanson E, et al. 2021. eLife. 10:00. PubMed
Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
NULL, et al. 2022. Cell. 185:916. PubMed
Goc J, et al. 2021. Cell. 184:5015. PubMed

RRID

AB_2629573 (BioLegend Cat. No. 300467)
AB_2629574 (BioLegend Cat. No. 300468)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

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Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 07/12/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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