Purified anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_UCHT1_Purified_021805
Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
  • 1_UCHT1_Purified_021805
    Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
  • 2_UCHT1_PURE_CD3_Antibody_IHC-F_090718
    Human frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD3 antibody (clone UCHT1, red) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053, red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Cat # Size Price Quantity Avail. Save
300401 25 µg £14
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300402 100 µg £27
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IP, Activ, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
Product Citations
  1. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  2. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  3. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  4. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  5. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  6. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  7. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  8. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  9. Hartmann FJ, et al. 2019. Cell Rep. 28:819. PubMed
  10. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  11. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  12. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  13. Wright CC, et al. 2017. Infect Immun. 85:e00131. PubMed
  14. Silva–Cardoso SC, et al. 2017. J Immunol. 199:253. PubMed
  15. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  16. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  17. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  18. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  19. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  20. Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
  21. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  22. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  23. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  24. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  25. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  26. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  27. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  28. Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed
  29. Oda H, et al. 2019. Front Immunol. 10:479. PubMed
  30. Yamaguchi K, et al. 2018. Cancer Sci. 109:3032. PubMed
  31. Michlmayr D, et al. 2020. Cell Reports. 31(4):107569. PubMed
  32. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  33. Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed
  34. Thompson A, et al. 2004. J Immunol. 173:1671. PubMed
  35. Guerrero A, et al. 2012. J Immunol. 190:27. PubMed
  36. Laurent A, et al. 2014. PLoS One. 9:103683. PubMed
  37. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  38. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  39. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  40. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  41. Schwabenland M, et al. 2021. Immunity. . PubMed
RRID
AB_314055 (BioLegend Cat. No. 300401)
AB_314056 (BioLegend Cat. No. 300402)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/17/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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