- 6B11 (See other available formats)
- Regulatory Status
- Other Names
- TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.Product Details
- Human, Cross-Reactivity: Rhesus, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.
(PubMed link indicates BioLegend citation)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
AB_2814261 (BioLegend Cat. No. 342929)
AB_2814262 (BioLegend Cat. No. 342930)
- Invariant CDR3 region of TCR Vα24-JαQ
Invariant NKT cells
- Glycolipid loaded CD1d
- Cell Type
- NKT cells
- Biology Area
- Molecular Family
- Antigen References
- Thomas SY, et al. 2003. J. Immunol. 171:2571.
- Exley MA, et al. 2008. Eur. J. Immunol. 38:1756.
- Montoya CJ, et al. 2007. Immunology. 122:1.
- Gansuvd B, et al. 2003. J. Immunol. 171:2904.
- Gene ID
- 28659 View all products for this Gene ID
- View information about TCR V alpha 24-J alpha 18 on UniProt.org
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