PE/Cyanine7 anti-human TCR Vα24-Jα18 (iNKT cell) Antibody

Pricing & Availability
Clone
6B11 (See other available formats)
Regulatory Status
RUO
Other Names
TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6B11_PECy7_TCRVa24-Ja18_Antibody_FC_1_062413
Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vα24-Jα18 (clone 6B11, top) PE/Cyanine7 or mouse IgG1, κ PE/Cyanine7 isotype control (bottom).
  • 6B11_PECy7_TCRVa24-Ja18_Antibody_FC_1_062413
    Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vα24-Jα18 (clone 6B11, top) PE/Cyanine7 or mouse IgG1, κ PE/Cyanine7 isotype control (bottom).
  • 6B11_PECy7_TCRVa24-Ja18_Antibody_FC_2_062413
Compare all formats See PE/Cyanine7 spectral data
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342911 25 tests £85
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342912 100 tests £221
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Description

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Product Citations
  1. Janeczek K, et al. 2023. Front Immunol. 14:1073788. PubMed
  2. Rosain J, et al. 2023. Cell. 186:621. PubMed
  3. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  4. Khurana P, et al. 2021. Front Immunol. 12:700374. PubMed
RRID
AB_2562229 (BioLegend Cat. No. 342911)
AB_2562230 (BioLegend Cat. No. 342912)

Antigen Details

Structure
Invariant CDR3 region of TCR Vα24-JαQ
Distribution

Invariant NKT cells

Ligand/Receptor
Glycolipid loaded CD1d
Cell Type
NKT cells
Biology Area
Immunology
Molecular Family
TCRs
Antigen References
  1. Thomas SY, et al. 2003. J. Immunol. 171:2571.
  2. Exley MA, et al. 2008. Eur. J. Immunol. 38:1756.
  3. Montoya CJ, et al. 2007. Immunology. 122:1.
  4. Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID
28659 View all products for this Gene ID
UniProt
View information about TCR Valpha24-Jalpha18 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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