Brilliant Violet 421™ anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
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Product Citations
publications
GK1.5_BV421_2_032911
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD4 (clone GK1.5) Brilliant Violet 421™ (left) or rat IgG2b, κ Brilliant Violet 421™ isotype control (right).
  • GK1.5_BV421_2_032911
    C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD4 (clone GK1.5) Brilliant Violet 421™ (left) or rat IgG2b, κ Brilliant Violet 421™ isotype control (right).
  • GK1.5_BV421_CD4_Antibody_IF_012914
    C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD4 (clone GK1.5) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone 6D5) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
100437 125 µL £103.00
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100443 50 µg £157.00
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100438 500 µL £233.00
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, and immunoprecipitation1,2. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
Product Citations
  1. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  2. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  3. Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed
  4. Goswami M, et al. 2019. Biomed Opt Express. 10:151. PubMed
  5. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  6. Bennion BG, et al. 2019. J Virol. 93. PubMed
  7. Silva DA, et al. 2019. Nature. 565:186. PubMed
  8. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  9. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
  10. Annu K, et al. 2020. Sci Rep. 10:2359. PubMed
  11. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  12. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  13. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  14. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  15. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  16. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  17. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  18. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  19. Shinohara M 2015. J Immunol. 194:5595. PubMed
  20. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  21. Gorman M, et al. 2016. J Virol. 90: 8212 - 8225. PubMed
  22. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  23. Li J, et al. 2020. Elife. 9:00. PubMed
  24. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  25. Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
  26. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
RRID
AB_10900241 (BioLegend Cat. No. 100437)
AB_2562557 (BioLegend Cat. No. 100443)
AB_11203718 (BioLegend Cat. No. 100438)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 02/18/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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