- 4B4-1 (See other available formats)
- Regulatory Status
- VI C-7
- Other Names
- 4-1BB, ILA, CD137, TNFRSF9
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.Product Details
- Verified Reactivity
- Reported Reactivity
- Chimpanzee, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Ectodomain of recombinant human 4-1BB fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
(PubMed link indicates BioLegend citation)
- Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
- Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
- Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
- Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
AB_528738 (BioLegend Cat. No. 309806)
- TNFR superfamily, type I transmembrane protein, 30 kD
Activated T cells
- T cell costimulation
- 4-1BB ligand
- Cell Type
- T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.
- Gene ID
- 3604 View all products for this Gene ID
- View information about CD137 on UniProt.org
Related Pages & Pathways
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other FormatsView All CD137 Reagents Request Custom Conjugation
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD137 (4-1BB)
PE anti-human CD137 (4-1BB)
Biotin anti-human CD137 (4-1BB)
PE/Cyanine5 anti-human CD137 (4-1BB)
APC anti-human CD137 (4-1BB)
PerCP/Cyanine5.5 anti-human CD137 (4-1BB)
Alexa Fluor® 700 anti-human CD137 (4-1BB)
PE/Cyanine7 anti-human CD137 (4-1BB)
Brilliant Violet 421™ anti-human CD137 (4-1BB)
APC/Cyanine7 anti-human CD137 (4-1BB)
Brilliant Violet 605™ anti-human CD137 (4-1BB)
Alexa Fluor® 647 anti-human CD137 (4-1BB)
PE/Dazzle™ 594 anti-human CD137 (4-1BB)
Brilliant Violet 650™ anti-human CD137 (4-1BB)
Brilliant Violet 711™ anti-human CD137 (4-1BB)
APC/Fire™ 750 anti-human CD137 (4-1BB)
TotalSeq™-A0355 anti-human CD137 (4-1BB)
TotalSeq™-B0355 anti-human CD137 (4-1BB)
TotalSeq™-C0355 anti-human CD137 (4-1BB)
Ultra-LEAF™ Purified anti-human CD137 (4-1BB)
Brilliant Violet 750™ anti-human CD137 (4-1BB)
TotalSeq™-D0355 anti-human CD137 (4-1BB)