APC/Fire™ 750 anti-human HLA-DR Antibody

Pricing & Availability
Clone
LN3 (See other available formats)
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
LN3_APCFire750_HLA-DR_Antibody_062619
Human peripheral blood lymphocytes were stained with HLA-DR (clone LN3) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
  • LN3_APCFire750_HLA-DR_Antibody_062619
    Human peripheral blood lymphocytes were stained with HLA-DR (clone LN3) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
327023 25 tests £101
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327024 100 tests £212
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Description

The LN3 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
human PBL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen sections and formalin-fixed paraffin-embedded sections1, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Marder RJ, et al. 1985. Lab. Invest. 52:497.
  2. Norton AJ and Isaacson PG. 1987. Am. J. Pathol. 128:225.
  3. Hua ZX, et al. 1998. Hum. Pathol. 29(12):1441.

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leuk. Biol. 48:138.
3. Edwards J, et al. 1985. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/26/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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