APC anti-human Tim-4 Antibody

Pricing & Availability
9F4 (See other available formats)
Regulatory Status
Other Names
SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)
Mouse IgG1, κ
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Product Citations
Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 9F4_APC_Tim-4_Antibody_FC_121114
    Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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354007 25 tests £111
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354008 100 tests £256
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The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, Tim-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The Tim-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to Tim-1-triggered Th2 responsiveness.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human TIM-4- IgV Fc, fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 9F4 recognizes the FG loop of human Tim-41.

Application References

(PubMed link indicates BioLegend citation)
  1. Kobayashi N, et al. 2007. Immunity 27:927.
Product Citations
  1. Jin S, et al. 2020. Cell Res. 30:950. PubMed
AB_2564543 (BioLegend Cat. No. 354007)
AB_2564544 (BioLegend Cat. No. 354008)

Antigen Details

Type 1 transmembrane glycoprotein and mucin domain containing protein 4.

Splenic CD14+, macrophages, immature DC.

Tim-1 ligation to Tim-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Phosphatiylserine is the receptor of Tim-4, and Tim-4 is a ligand of Tim-1.
Cell Type
Dendritic cells, Macrophages
Biology Area
Antigen References

1. Kobayashi N, et al. 2007. Immunity 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.

Gene ID
91937 View all products for this Gene ID
View information about Tim-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/16/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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