PE anti-human Tim-4 Antibody

Pricing & Availability
9F4 (See other available formats)
Regulatory Status
Other Names
SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 9F4_PE_011712
    Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
354003 25 tests £105
Check Availability

Need larger quantities of this item?
Request Bulk Quote
354004 100 tests £247
Check Availability

Need larger quantities of this item?
Request Bulk Quote

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, Tim-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The Tim-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to Tim-1-triggered Th2 responsiveness.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human TIM-4- IgV Fc, fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 9F4 recognizes the FG loop of human Tim-41.

Application References

(PubMed link indicates BioLegend citation)
  1. Kobayashi N, et al. 2007. Immunity 27:927.
Product Citations
  1. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  2. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  3. Yano H, et al. 2017. Med Mol Morphol. . 10.1007/s00795-017-0164-9. PubMed
AB_11124345 (BioLegend Cat. No. 354003)
AB_11124105 (BioLegend Cat. No. 354004)

Antigen Details

Type 1 transmembrane glycoprotein and mucin domain containing protein 4.

Splenic CD14+, macrophages, immature DC.

Tim-1 ligation to Tim-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Phosphatiylserine is the receptor of Tim-4, and Tim-4 is a ligand of Tim-1.
Cell Type
Dendritic cells, Macrophages
Biology Area
Antigen References

1. Kobayashi N, et al. 2007. Immunity 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.

Gene ID
91937 View all products for this Gene ID
View information about Tim-4 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05/15/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human Tim-4

  • PE anti-human Tim-4

  • APC anti-human Tim-4

  • PE/Cyanine7 anti-human Tim-4

  • TotalSeq™-A0428 anti-human Tim-4

  • TotalSeq™-C0428 anti-human Tim-4

  • TotalSeq™-B0428 anti-human Tim-4


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account