Alexa Fluor® 647 anti-mouse Notch 3 Antibody

Pricing & Availability
HMN3-133 (See other available formats)
Regulatory Status
Other Names
Neurogenic locus notch homolog protein 3, Notch-3, CADASIL
Armenian Hamster IgG
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Product Citations
FN3/CHO (Notch-3 transfected) cells stained with HMN3-133 Alexa Fluor® 647
  • HMN3-133_A647_100908.jpg
    FN3/CHO (Notch-3 transfected) cells stained with HMN3-133 Alexa Fluor® 647
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130511 25 µg £101
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130512 100 µg £238
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The Notch receptors are highly conserved from invertebrates to mammals.While Notch1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences.The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus.This translocation triggers a series of signaling process.Notch 3 is primarily expressed in adult arterial smooth muscle cells.Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Armenian Hamster
Notch 3-Fc fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Moriyama Y, et al. 2008. Int J Immunology 20:763
Product Citations
  1. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
AB_2151249 (BioLegend Cat. No. 130511)
AB_2151248 (BioLegend Cat. No. 130512)

Antigen Details

Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Notch 3 is primarily expressed in vascular smooth muscle cells, also found in proliferating neuroepithelium.
Critical role in the structural integrity of distal resistance artieries. Mutations in the Notch 3 receptor on vascular smooth muscles cells is associated with an inherited neurodegenerative disease CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopthy)
Jag1,2; Delta 1,2
Cell Type
Epithelial cells
Biology Area
Antigen References
1. Ehebauer ME et al. 2006. Biochem J 392:13
2. Shimizu K et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K et al. 2007. Nature Immunol 8:451
4. Bellavia D et al. 2008. 27:5092
5. Louvi A et al. Dev Neurosci. 2006. 28:5
Gene ID
18131 View all products for this Gene ID
View information about Notch 3 on

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse Notch 3

  • PE anti-mouse Notch 3

  • Alexa Fluor® 647 anti-mouse Notch 3


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