Alexa Fluor® 488 anti-IRF4 Antibody

Pricing & Availability
Clone
IRF4.3E4 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 4
Isotype
Rat IgG1, κ
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Product Citations
publications
IRF4.3E4_AF488_IRF4_Antibody_FC_022814
PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set. Cells were then stained with IRF4 (clone IRF4.3E4) Alexa Fluor® 488 (open histogram with solid line: fresh cells, filled histogram: stimulated cells) or rat IgG1, κ Alexa Fluor® 488 isotype control (dash line: fresh cell, dot line: stimulated cells).
  • IRF4.3E4_AF488_IRF4_Antibody_FC_022814
    PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set. Cells were then stained with IRF4 (clone IRF4.3E4) Alexa Fluor® 488 (open histogram with solid line: fresh cells, filled histogram: stimulated cells) or rat IgG1, κ Alexa Fluor® 488 isotype control (dash line: fresh cell, dot line: stimulated cells).
See Alexa Fluor® 488 spectral data
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646405 25 µg £92.00
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646406 100 µg £215.00
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Description

The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
GST fusion protein containing C-terminal of murine IRF4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zheng Y, et al. 2009. Nature 458:351
  2. Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed
Product Citations
  1. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  2. Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
  3. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  4. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  5. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  6. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
RRID
AB_2563266 (BioLegend Cat. No. 646405)
AB_2563267 (BioLegend Cat. No. 646406)

Antigen Details

Structure
Molecular weight is approximately 51 kD.
Distribution

IRF4 is reported to be expressed exclusively in the immune system.

Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lu R. 2008. Trends Immunol 29:487.

Gene ID
3662 View all products for this Gene ID
UniProt
View information about IRF4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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