Purified anti-mouse Notch 3 Antibody

Pricing & Availability
Clone
HMN3-133 (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic locus notch homolog protein 3, Notch-3, CADASIL
Isotype
Armenian Hamster IgG
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Product Citations
publications
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130502 500 µg £229.00
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Description

The Notch receptors are highly conserved from invertebrates to mammals.While Notch1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences.The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus.This translocation triggers a series of signaling process.Notch 3 is primarily expressed in adult arterial smooth muscle cells.Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Notch 3-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Moriyama Y, et al. 2008. Int J Immunology 20:763
  2. Ghosh S, et al. 2011. J. Biol Chem. 286:22678. PubMed.
  3. Romano RA, et al. 2012. Development 139:772. PubMed.
Product Citations
  1. Zhang H et al. 2017. eLife. 6 pii: e30126. PubMed
  2. Ghosh S, et al. 2011. J Biol Chem. 286:22678. PubMed
  3. Romano R, et al. 2012. Development. 139:772. PubMed
RRID
AB_1227735 (BioLegend Cat. No. 130502)

Antigen Details

Structure
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution

Notch 3 is primarily expressed in vascular smooth muscle cells, also found in proliferating neuroepithelium.

Function
Critical role in the structural integrity of distal resistance artieries. Mutations in the Notch 3 receptor on vascular smooth muscles cells is associated with an inherited neurodegenerative disease CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopthy)
Ligand/Receptor
Jag1,2; Delta 1,2
Cell Type
Epithelial cells
Biology Area
Immunology
Antigen References

1. Ehebauer ME, et al. 2006. Biochem J 392:13
2. Shimizu K, et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K, et al. 2007. Nature Immunol 8:451
4. Bellavia D, et al. 2008. 27:5092
5. Louvi A, et al. Dev Neurosci. 2006. 28:5

Gene ID
18131 View all products for this Gene ID
UniProt
View information about Notch 3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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