- 7C2C34 (See other available formats)
- Regulatory Status
- Other Names
- Spleen focus forming virus (SFFV) proviral integration oncogene, Sfpi1, Transcription factor PU.1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1
- Rat IgG2a, κ
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- Product Citations
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SPI1 is a transcription factor that belongs to the E26-transformation-specific (ETS) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during the differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as malignancy. The overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele shows decreased SPI1 expression and develops acute myeloid leukaemia (AML), which suggests that SPI1 plays a role in oncogenesis.Product Details
- Antibody Type
- Host Species
- Full length SPI1 recombinant protein expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Blue Laser (488 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
AB_2629611 (BioLegend Cat. No. 681305)
AB_2629612 (BioLegend Cat. No. 681306)
- 272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.
- SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.
- SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmele P, et al. 2010. Cancer Res. 70:6757.
- Gene ID
- 20375 View all products for this Gene ID
- View information about SPI1 on UniProt.org
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