Alexa Fluor® 488 anti-mouse CD169 (Siglec-1) Antibody

Pricing & Availability
Clone
3D6.112 (See other available formats)
Other Names
Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)
Isotype
Rat IgG2a, κ
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Product Citations
publications
3D6.112_A488_CD169_Antibody_IF_110315
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of CD169 (clone 3D6.112) Alexa Fluor® 488 (green), 5 µg/ml of F4/80 (clone BM8) Alexa Fluor® 647 (red), 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 594 (yellow), and 5 µg/ml of CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ (Blue) overnight at 4°C. The image was captured with a 10X objective.
  • 3D6.112_A488_CD169_Antibody_IF_110315
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of CD169 (clone 3D6.112) Alexa Fluor® 488 (green), 5 µg/ml of F4/80 (clone BM8) Alexa Fluor® 647 (red), 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 594 (yellow), and 5 µg/ml of CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ (Blue) overnight at 4°C. The image was captured with a 10X objective.
  • 3D6dot112_A488_CD169_Antibody_2_121018
    Dissected C57/B6 mouse spleen was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 5 µg/mL of anti-mouse CD3 (clone 17A2) Alexa Fluor® 647 (red) and 5 µg/mL of anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 594 (blue) and 5 µg/mL of anti-mouse CD169 (clone 3D6.112) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
  • 3D6dot112_A488_CD169_Antibody_3_050219
    Formalin-fixed, 400 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with CD21/35 (CR2/CR1) (clone 7E9) Alexa Fluor® 594 (red), CD169 (Siglec-1) (clone 3D6.112) Alexa Fluor® 488 (green), and CD3 (clone 17A2) Alexa Fluor® 647 (blue) all at 5 µg/mL, optically cleared, then analyzed at 235 µm imaging depth on a confocal microscope. Scale bar: 100 µm. Watch the video.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
142419 100 µg £218
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Description

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8+ T cells and invariant NK cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Native Sialoadhesin from spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
3-D IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 0.1 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
  2. Chtanova T, et al. 2008. Immunity 29:487.
  3. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
Product Citations
  1. Desbien A, et al. 2016. J Immunol. 197:4351-4359. PubMed
  2. Sokol CL 2018. Immunity. 49:449. PubMed
Publication Library
RRID
AB_2566436 (BioLegend Cat. No. 142419)

Antigen Details

Structure
Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution

Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.

Function
Adhesion.
Ligand/Receptor
PSGL-1, CD43, CD206 and CD227.
Cell Type
Macrophages
Biology Area
Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References

1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.

Gene ID
20612 View all products for this Gene ID
UniProt
View information about CD169 on UniProt.org

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Go To Top Version: 2    Revision Date: 05/02/2019

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