- M5/114.15.2 (See other available formats)
- Regulatory Status
- Other Names
- MHC class II
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.Product Details
- Antibody Type
- Host Species
- Activated C57BL/6 mouse spleen cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
- Additional Product Notes
Maxpar® is a registered trademark of Fluidigm Inc.
(PubMed link indicates BioLegend citation)
- Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
- Viville S, et al. 1993. Cell 72:635. (IHC)
- Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
- Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
- Yamashita I, et al. 1993. Int. Immunol. 5:1139.
- Guo M, et al. 1995. Zygote 3:65. (IHC)
- Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
- Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
- Venanzi ES, et al. 2007. J. Immunol. 179:5693.
- Christensen SR, et al. 2006. Immunity 25:417. PubMed
- Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
- De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
- Kuns RD, et al. 2009. Blood 113:5999. PubMed
- Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
- Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
- Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_2563771 (BioLegend Cat. No. 107637)
- MHC class II
B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice
- Antigen presentation
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
- Gene ID
- 14961 View all products for this Gene ID 14969 View all products for this Gene ID
- View information about I-A/I-E on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse I-A/I-E
FITC anti-mouse I-A/I-E
PE anti-mouse I-A/I-E
Purified anti-mouse I-A/I-E
PE/Cyanine5 anti-mouse I-A/I-E
APC anti-mouse I-A/I-E
Alexa Fluor® 488 anti-mouse I-A/I-E
Alexa Fluor® 647 anti-mouse I-A/I-E
Pacific Blue™ anti-mouse I-A/I-E
Alexa Fluor® 700 anti-mouse I-A/I-E
PerCP/Cyanine5.5 anti-mouse I-A/I-E
PerCP anti-mouse I-A/I-E
APC/Cyanine7 anti-mouse I-A/I-E
PE/Cyanine7 anti-mouse I-A/I-E
Brilliant Violet 421™ anti-mouse I-A/I-E
Brilliant Violet 510™ anti-mouse I-A/I-E
Purified anti-mouse I-A/I-E (Maxpar® Ready)
Brilliant Violet 605™ anti-mouse I-A/I-E
Brilliant Violet 650™ anti-mouse I-A/I-E
Brilliant Violet 711™ anti-mouse I-A/I-E
Brilliant Violet 785™ anti-mouse I-A/I-E
PE/Dazzle™ 594 anti-mouse I-A/I-E
Alexa Fluor® 594 anti-mouse I-A/I-E
APC/Fire™ 750 anti-mouse I-A/I-E
TotalSeq™-A0117 anti-mouse I-A/I-E
Ultra-LEAF™ Purified anti-mouse I-A/I-E
TotalSeq™-B0117 anti-mouse I-A/I-E
TotalSeq™-C0117 anti-mouse I-A/I-E
Spark Blue™ 550 anti-mouse I-A/I-E
PE/Fire™ 640 anti-mouse I-A/I-E
Spark YG™ 581 anti-mouse I-A/I-E
PE/Fire™ 810 anti-mouse I-A/I-E
Spark UV™ 387 anti-mouse I-A/I-E