Brilliant Violet 421™ Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Poly4064 (See other available formats)
Regulatory Status
Donkey Polyclonal Ig
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Product Citations
293E cells were stained by purified anti-Tubulin-gamma (clone Poly6209) and labeled with BV421 anti-rabbit-IgG (clone Poly4064 (blue)). Nuclei were stained with propidium iodide (red).
  • Poly4064_BV421_IgG_Antibody_IF_012213
    293E cells were stained by purified anti-Tubulin-gamma (clone Poly6209) and labeled with BV421 anti-rabbit-IgG (clone Poly4064 (blue)). Nuclei were stained with propidium iodide (red).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
406410 125 µL 148,00€
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This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICC - Quality tested
FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, the suggested usage of this reagent is a 1:20-1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  2. Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed
  3. Henry GH et al. 2018. Cell reports. 25(12):3530-3542 . PubMed
  4. Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed
  5. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  6. Bohlen CJ et al. 2017. Neuron. 94(4):759-773 . PubMed
  7. Yamasaki T, et al. 2018. J Virol. 92:e01458. PubMed
  8. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  9. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  10. Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed
  11. Lamsoul I, et al. 2012. PLoS One. 7:e43798. PubMed
  12. Becker L, et al. 2013. PLoS One. 8:e59452. PubMed
  13. Onodera T, et al. 2015. Sci Rep. 5:16801. PubMed
  14. Cao W, et al. 2016. Nat Commun. 7:11687. PubMed
  15. Vogel R, Erez A, Altan-Bonnet G 2016. Nat Commun. 7: 12428. PubMed
  16. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  17. Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
  18. Madge P, et al. 2016. Sci Rep. 6:36012. PubMed
  19. Muto M, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016050496. PubMed
  20. Liddelow S, et al. 2017. Nature. 541:481-487. PubMed
AB_10897810 (BioLegend Cat. No. 406410)

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 02.09.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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