Cyanine3 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_Cyanine3_IgG_Antibody_050520
HeLa cells were fixed with ice cold methanol for six minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/mL of purified anti-Giantin (clone Poly19087) and followed by Cyanine3 Donkey anti-rabbit IgG (clone Poly4064) antibody (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • Poly4064_Cyanine3_IgG_Antibody_050520
    HeLa cells were fixed with ice cold methanol for six minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/mL of purified anti-Giantin (clone Poly19087) and followed by Cyanine3 Donkey anti-rabbit IgG (clone Poly4064) antibody (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
See Cyanine3 spectral data
Cat # Size Price Quantity Avail. Save
406402 100 µg 72,00€
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with Cyanine3 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Additional Product Notes

BioLegend is in the process of converting the name Cy3 to Cyanine3. The dye molecule remains the same, so you should expect the same quality and performance from our Cyanine3 products. Please contact Technical Service if you have any questions.

Product Citations
  1. Liu J, et al. 2019. Mol Med Rep. 20:33. PubMed
  2. Meinen S, et al. 2012. PLoS One. 7:e44148. PubMed
  3. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  4. Ali H, et al. 2014. J Control Release. 183:167. PubMed
  5. Rauner G, Barash I 2014. Exp Cell Res. 328:186. PubMed
  6. Hu J, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.72. PubMed
  7. Han JK, et al. 2021. eLife. 10:00. PubMed
RRID
AB_893532 (BioLegend Cat. No. 406402)

Related FAQs

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Go To Top Version: 4    Revision Date: 05.05.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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