FITC Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_FITC_IgG_Antibody_IF_032613
Hela cells were stained with anti-GRASP65 (Poly6210) and secondarily labeled with FITC donkey anti-rabbit IgG (clone Poly4064).
  • Poly4064_FITC_IgG_Antibody_IF_032613
    Hela cells were stained with anti-GRASP65 (Poly6210) and secondarily labeled with FITC donkey anti-rabbit IgG (clone Poly4064).
See FITC spectral data
Cat # Size Price Quantity Avail. Save
406403 100 µg 56,00€
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Tang H, et al. 2017. J Immunol. 199:570. PubMed
  2. Mohamadi F, et al. 2019. Biomed Rep. 10:183. PubMed
  3. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  4. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  5. Yang Y, et al. 2020. J Clin Invest. 130:5508. PubMed
  6. Zeng G, et al. 2011. J Immunol. 187:190. PubMed
  7. Ryzhov S, et al. 2012. J Pharmacol Exp Ther. 341:764. PubMed
  8. Tittarelli A, et al. 2014. J Immunol. 192:1313. PubMed
  9. Wong C, et al. 2014. J Leukoc Biol. 95:631. PubMed
  10. Janczy J, et al. 2014. J Immunol. 193:5190. PubMed
  11. Nicholas D, et al. 2015. Mol Cell Proteomics. 14:15. PubMed
  12. Yang J, et al. 2020. Nature. 586:572. PubMed
  13. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  14. Sun Z, et al. 2021. eLife. 10:00. PubMed
  15. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
RRID
AB_893531 (BioLegend Cat. No. 406403)

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Go To Top Version: 5    Revision Date: 03.16.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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