DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_D488_100410
293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).
  • Poly4064_D488_100410
    293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).
Cat # Size Price Quantity Avail. Save
406404 100 µg 72€
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with DyLight™ 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, IF

Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight 488  has a maximum absorption of 493nm and a maximum emission of 518 nm (similar to Alexa Fluor® 488 or FITC).

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Aronovich A, et al. 2013. Blood. 122:2562. PubMed
  2. Chapman N, et al. 2013. J Immunol. 191:6208. PubMed
  3. Granato A, et al. 2014. J Immunol. 192:5761. PubMed
  4. Chen Y, et al. 2014. Blood. 124:936. PubMed
  5. Beam J, et al. 2015. J Biol Chem. 290: 23371-23384. PubMed
  6. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  7. Zheng Q, et al. 2016. Sci Rep. 6: 33489. PubMed
  8. Liu R, et al. 2017. PLoS One. 10.1371/journal.pone.0170666. PubMed
  9. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  10. Parvatiyar K, et al. 2018. Nat Commun. 9:2770. PubMed
  11. Laugsch M et al. 2019. Cell stem cell. 24(5):736-752 . PubMed
Publication Library
RRID
AB_1575130 (BioLegend Cat. No. 406404)

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Go To Top Version: 3    Revision Date: 02.09.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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