- M5/114.15.2 (See other available formats)
- Regulatory Status
- Other Names
- MHC class II
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.Product Details
- Antibody Type
- Host Species
- Activated C57BL/6 mouse spleen cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
- Application Notes
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
(PubMed link indicates BioLegend citation)
- Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
- Viville S, et al. 1993. Cell 72:635. (IHC)
- Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
- Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
- Yamashita I, et al. 1993. Int. Immunol. 5:1139.
- Guo M, et al. 1995. Zygote 3:65. (IHC)
- Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
- Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
- Venanzi ES, et al. 2007. J. Immunol. 179:5693.
- Christensen SR, et al. 2006. Immunity 25:417. PubMed
- Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
- De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
- Kuns RD, et al. 2009. Blood 113:5999. PubMed
- Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
- Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
- Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
- Product Citations
AB_2616728 (BioLegend Cat. No. 107651)
AB_2616729 (BioLegend Cat. No. 107652)
- MHC class II
B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice
- Antigen presentation
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
- Gene ID
- 14961 View all products for this Gene ID 14969 View all products for this Gene ID
- View information about I-A/I-E on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE/Cyanine5 anti-mouse I-A/I-E
APC anti-mouse I-A/I-E
Alexa Fluor® 488 anti-mouse I-A/I-E
Alexa Fluor® 647 anti-mouse I-A/I-E
Pacific Blue™ anti-mouse I-A/I-E
Brilliant Violet 510™ anti-mouse I-A/I-E
Alexa Fluor® 594 anti-mouse I-A/I-E
Biotin anti-mouse I-A/I-E
FITC anti-mouse I-A/I-E
PE anti-mouse I-A/I-E
Purified anti-mouse I-A/I-E
Spark Blue™ 550 anti-mouse I-A/I-E
Alexa Fluor® 700 anti-mouse I-A/I-E
PerCP/Cyanine5.5 anti-mouse I-A/I-E
PerCP anti-mouse I-A/I-E
APC/Cyanine7 anti-mouse I-A/I-E
PE/Cyanine7 anti-mouse I-A/I-E
Purified anti-mouse I-A/I-E (Maxpar® Ready)
Brilliant Violet 605™ anti-mouse I-A/I-E
TotalSeq™-A0117 anti-mouse I-A/I-E
Ultra-LEAF™ Purified anti-mouse I-A/I-E
TotalSeq™-C0117 anti-mouse I-A/I-E
TotalSeq™-B0117 anti-mouse I-A/I-E
PE/Fire™ 640 anti-mouse I-A/I-E
Spark YG™ 581 anti-mouse I-A/I-E
Brilliant Violet 421™ anti-mouse I-A/I-E
PE/Fire™ 810 anti-mouse I-A/I-E
Brilliant Violet 650™ anti-mouse I-A/I-E
Brilliant Violet 711™ anti-mouse I-A/I-E
Brilliant Violet 785™ anti-mouse I-A/I-E
PE/Dazzle™ 594 anti-mouse I-A/I-E