APC/Cyanine7 anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
Ave. Rating
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Product Citations
publications
PC61_APCCy7_110308.jpg
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 APC/Cyanine7
  • PC61_APCCy7_110308.jpg
    Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 APC/Cyanine7
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
102025 25 µg 84€
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102026 100 µg 212€
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  2. Chan S, et al. 2018. PLoS One. 13:e0207619. PubMed
  3. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  4. Eremenko E, et al. 2019. EBioMedicine. 43:424. PubMed
  5. Li C, et al. 2018. Cell. 174:285. PubMed
  6. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  7. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  8. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  9. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  10. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  11. Olguín JE, et al. 2018. J Cancer. 9:239. PubMed
  12. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  13. Di Luccia B, et al. 2020. Cell Host & Microbe. 27(6):899-908. PubMed
  14. Randall K, et al. 2011. J Exp Med. 208:2305. PubMed
  15. Rio R, et al. 2011. J Immunol. 186:382. PubMed
  16. Morawski P, et al. 2013. J Biol Chem. 288:24494. PubMed
  17. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  18. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  19. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  20. Long C, et al. 2016. Toxicol Sci. 152: 85 - 98. PubMed
  21. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  22. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  23. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
RRID
AB_830744 (BioLegend Cat. No. 102025)
AB_830745 (BioLegend Cat. No. 102026)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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