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Ultra-LEAF™ Purified anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
A17188B (See other available formats)
Regulatory Status
RUO
Other Names
PD1, PDCD1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17188B_ULEAF_CD279_Antibody_100719.png
Recombinant hPD-1 binds to hPD-L1. Anti-human PD-1 Antibody (clone A17188B, purple squares) inhibits the binding in a dose-dependent manner. The ND50 =0.1 – 0.5 µg/mL.
  • A17188B_ULEAF_CD279_Antibody_100719.png
    Recombinant hPD-1 binds to hPD-L1. Anti-human PD-1 Antibody (clone A17188B, purple squares) inhibits the binding in a dose-dependent manner. The ND50 =0.1 – 0.5 µg/mL.
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621603 100 µg 216€
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621604 1 mg 572€
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Description

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD279 protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking hPD-1 and hPD-L1 binding in the presence of 0.8 µg/mL hPD-1 Biotin. ND50 range: 0.1– 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.

Product Citations
  1. Murakami H, et al. 2024. Front Immunol. 1363568:15. PubMed
RRID
AB_2820104 (BioLegend Cat. No. 621603)
AB_2820105 (BioLegend Cat. No. 621604)

Antigen Details

Structure
55 kD type I transmembrane protein
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling and co-stimulation (co-inhibition)
Interaction
SHP-1 and SHP-2
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887
  2. Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10-07-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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