PE/Fire™ 640 anti-human CD279 (PD-1) Antibody

Pricing & Availability
A17188B (See other available formats)
Regulatory Status
Other Names
Mouse IgG2b, κ
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Product Citations
PHA-stimulated (three days) human peripheral blood lymphocytes were stained with anti-human CD3 PE and anti-human CD279 (PD-1) (clone A17188B) PE/Fire™ 640 (left) or CD3 PE only (right).
  • A17188B_PE_F640_CD279_Antibody_031522
    PHA-stimulated (three days) human peripheral blood lymphocytes were stained with anti-human CD3 PE and anti-human CD279 (PD-1) (clone A17188B) PE/Fire™ 640 (left) or CD3 PE only (right).
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621623 25 tests 141€
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621624 100 tests 306€
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Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant human CD279 protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.

AB_2910490 (BioLegend Cat. No. 621623)
AB_2910490 (BioLegend Cat. No. 621624)

Antigen Details

55 kD type I transmembrane protein

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Signaling and co-stimulation (co-inhibition)
SHP-1 and SHP-2
PD-L1 (CD274) and PD-L2 (CD273)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887
  2. Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID
5133 View all products for this Gene ID
View information about CD279 on

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Go To Top Version: 1    Revision Date: 12-14-2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • FITC anti-human CD279 (PD-1)

  • PerCP/Cyanine5.5 anti-human CD279 (PD-1)

  • PE/Cyanine7 anti-human CD279 (PD-1)

  • TotalSeq™-D1169 anti-human CD279 (PD-1)

  • APC/Fire™ 810 anti-human CD279 (PD-1)

  • PE/Fire™ 640 anti-human CD279 (PD-1)

  • PE/Fire™ 700 anti-human CD279 (PD-1)

  • PE/Fire™ 810 anti-human CD279 (PD-1)


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