Ultra-LEAF™ Purified anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
29F.1A12 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, Programmed Death-1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
29F-1A12_PURE_CD279_Antibody_102518
Con A and IL-2 stimulated mouse splenocytes (day 3) were stained with CD3ε APC and purified CD279 (clone 29F.1A12, left) or rat IgG2a, κ isotype control (right) followed by FITC anti-rat IgG.
  • 29F-1A12_PURE_CD279_Antibody_102518
    Con A and IL-2 stimulated mouse splenocytes (day 3) were stained with CD3ε APC and purified CD279 (clone 29F.1A12, left) or rat IgG2a, κ isotype control (right) followed by FITC anti-rat IgG.
Compare all formats
Cat # Size Price Quantity Check Availability Save
135245 100 µg 88€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
135246 1 mg 120€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
135247 5 mg 432€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
135248 25 mg 1127€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
135249 50 mg 1884€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
135250 100 mg 2707€
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This product is eligible for a 40% discount! Promotion valid until September 30th, 2022. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.

Application References
  1. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  2. Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
  3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
  4. Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2783088 (BioLegend Cat. No. 135245)
AB_2783089 (BioLegend Cat. No. 135246)
AB_2783090 (BioLegend Cat. No. 135247)
AB_2783091 (BioLegend Cat. No. 135248)
AB_2783092 (BioLegend Cat. No. 135249)
AB_2783093 (BioLegend Cat. No. 135250)

Antigen Details

Structure
A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.

Function
Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor
B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, T cells
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10-25-2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE/Dazzle™ 594 anti-mouse CD279 (PD-1)

  • Alexa Fluor® 647 anti-mouse CD279 (PD-1)

  • Brilliant Violet 711™ anti-mouse CD279 (PD-1)

  • GoInVivo™ Purified anti-mouse CD279 (PD-1)

  • APC/Fire™ 750 anti-mouse CD279 (PD-1)

  • Brilliant Violet 510™ anti-mouse CD279 (PD-1)

  • Ultra-LEAF™ Purified anti-mouse CD279 (PD-1)

  • APC/Fire™ 810 anti-mouse CD279 (PD-1) Antibody

  • PE/Fire™ 810 anti-mouse CD279 (PD-1) Antibody

  • PE/Cyanine5 anti-mouse CD279 (PD-1)

  • Purified anti-mouse CD279 (PD-1)

  • PE anti-mouse CD279 (PD-1)

  • PerCP/Cyanine5.5 anti-mouse CD279 (PD-1)

  • APC anti-mouse CD279 (PD-1)

  • Biotin anti-mouse CD279 (PD-1)

  • FITC anti-mouse CD279 (PD-1)

  • PE/Cyanine7 anti-mouse CD279 (PD-1)

  • Brilliant Violet 421™ anti-mouse CD279 (PD-1)

  • Brilliant Violet 605™ anti-mouse CD279 (PD-1)

  • APC/Cyanine7 anti-mouse CD279 (PD-1)

  • Brilliant Violet 785™ anti-mouse CD279 (PD-1)

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account