PE/Cyanine7 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
17A2_PECy7_083105
C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
  • 17A2_PECy7_083105
    C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
100219 25 µg 84€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100220 100 µg 199€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  2. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  3. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  4. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  5. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  6. Schneider C, et al. 2018. Cell. 174:271. PubMed
  7. Page N, et al. 2018. Immunity. 48:937. PubMed
  8. Vacca M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01462. PubMed
  9. Xiao C, et al. 2021. Front Pharmacol. 12:721273. PubMed
  10. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  11. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  12. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  13. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  14. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  15. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  16. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  17. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  18. Chen S, et al. 2022. Cancer Discov. :. PubMed
  19. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  20. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  21. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  22. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  23. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  24. Feizi N, et al. 2021. Cell Death Dis. 12:1026. PubMed
  25. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  26. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  27. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  28. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
  29. Tian X, et al. 2022. Front Immunol. 13:875236. PubMed
  30. Patnode M, et al. 2013. J Biol Chem. 288:26533. PubMed
  31. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  32. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  33. Jia L, et al. 2022. Front Immunol. 13:897879. PubMed
  34. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  35. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  36. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  37. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  38. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
  39. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  40. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  41. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  42. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  43. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  44. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  45. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  46. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  47. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  48. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  49. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  50. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  51. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  52. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  53. Kulkarni R, et al. 2021. PLoS One. 16:e0257191. PubMed
  54. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  55. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  56. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  57. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  58. De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed
  59. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  60. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  61. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  62. Song P, et al. 2018. Int J Mol Med. 41:935. PubMed
  63. Yazlovitskaya EM, et al. 2021. J Cell Sci. 134:. PubMed
  64. Yang R, et al. 2022. J Adv Res. 35:259. PubMed
  65. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  66. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  67. M H, et al. 2016. Sci Rep. 6: 20588. PubMed
  68. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  69. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  70. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  71. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  72. Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
  73. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  74. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  75. Chen Z, et al. 2019. Mol Med Rep. 19:3210. PubMed
  76. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  77. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  78. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  79. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  80. Glasenapp A, et al. 2021. Circ Res. 129:369. PubMed
  81. Haase P, et al. 2020. Eur J Immunol. 50:558. PubMed
  82. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  83. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  84. Kung YJ, et al. 2022. J Biomed Sci. 29:57. PubMed
  85. Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed
  86. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  87. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  88. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  89. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  90. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  91. Eastman AE, et al. 2020. Cell Rep. 31:107804. PubMed
  92. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  93. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  94. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  95. Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed
  96. Li H, et al. 2022. iScience. 25:104481. PubMed
  97. Catrysse L, et al. 2021. Cell Rep. 36:109748. PubMed
  98. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  99. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  100. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
RRID
AB_1732068 (BioLegend Cat. No. 100219)
AB_1732057 (BioLegend Cat. No. 100220)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account