APC/Cyanine7 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
17A2_APCCyanine7_CD3_Antibody_031919_updated.png
C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD3 (clone 17A2) APC/Cyanine7 (left) or Rat IgG2b, ? APC/Cyanine7 isotype control (right).
  • 17A2_APCCyanine7_CD3_Antibody_031919_updated.png
    C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD3 (clone 17A2) APC/Cyanine7 (left) or Rat IgG2b, ? APC/Cyanine7 isotype control (right).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
100221 25 µg 76,00€
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100222 100 µg 176,00€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Product Citations
  1. Teater M, et al. 2018. Nat Commun. 9:222. PubMed
  2. Revathikumar P, et al. 2018. PLoS One. 13:e0193210. PubMed
  3. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
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  5. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  6. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  7. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  8. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  9. Durgeau A, et al. 2018. Nat Commun. 9:5097. PubMed
  10. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  11. Adane B, et al. 2019. Cell Rep. 27:238. PubMed
  12. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  13. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  14. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  15. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  16. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  17. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  18. Dokoshi T, et al. 2020. Cell Rep. 30:61. PubMed
  19. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  20. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  21. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  22. Zhu W, et al. 2019. FASEB J. 33:5208. PubMed
  23. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  24. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  25. Silver AC, et al. 2018. Heliyon. 4:e00579. PubMed
  26. Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed
  27. Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
  28. Spees A, et al. 2014. Infect Immun . 82:1692. PubMed
  29. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  30. Voss M, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L188 - L195. PubMed
  31. Wen J, et al. 2015. J Pharmacol Sci. 128: 116-124. PubMed
  32. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  33. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  34. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  35. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  36. Li X, et al. 2020. Nat Commun. 4.877777778. PubMed
  37. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  38. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  39. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  40. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  41. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  42. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  43. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  44. Shi L, et al. 2021. Immunity. . PubMed
RRID
AB_2057374 (BioLegend Cat. No. 100221)
AB_2242784 (BioLegend Cat. No. 100222)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03-19-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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