PerCP/Cyanine5.5 anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
6 reviews
Product Citations
publications
53-6dot7_PerCPCyanine55_CD8a_Antibody_102318
C57BL/6 mouse splenocytes were stained with CD3e FITC and CD8a (clone 53-6.7) PerCP/Cyanine5.5, or Rat IgG2a, ? PerCP/Cyanine5.5 isotype control (bottom).
  • 53-6dot7_PerCPCyanine55_CD8a_Antibody_102318
    C57BL/6 mouse splenocytes were stained with CD3e FITC and CD8a (clone 53-6.7) PerCP/Cyanine5.5, or Rat IgG2a, ? PerCP/Cyanine5.5 isotype control (bottom).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
100733 25 µg 64€
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100734 100 µg 176€
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, and immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 μm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
Product Citations
  1. Dekhtiarenko I, et al. 2013. J Immunol. 190:3399. PubMed
  2. Dutow P, et al. 2014. J Infect Dis. 209:1269. PubMed
  3. Hartwig S, et al. 2014. PLoS One. 9:90720. PubMed
  4. White C, et al. 2015. J Immunol. 194:697. PubMed
  5. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  6. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  7. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  8. Holvoet B, et al. 2015. Stem Cell Reports. 5: 1183-1195. PubMed
  9. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  10. Pilzecker B, et al. 2016. Nucleic Acids Res. 44: 4734 - 4744. PubMed
  11. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  12. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  13. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  14. Gorman M, et al. 2016. J Virol. 90: 8212 - 8225. PubMed
  15. Collinson-Pautz M, et al. 2016. PLoS One. 11:e0164547. PubMed
  16. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  17. Dekhtiarenko I, et al. 2016. PLoS Pathog. 12:e1006072. PubMed
  18. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  19. Borkner L, et al. 2017. J Immunol. 10.4049/jimmunol.1602115. PubMed
  20. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  21. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
  22. Solanki A, et al. 2018. Development. 145. PubMed
  23. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  24. Shi R, et al. 2019. J Cancer. 10:367. PubMed
  25. Novince CM, et al. 2017. Sci Rep. 7:5747. PubMed
  26. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  27. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  28. Karandikar SH, et al. 2019. Anal Chem. 91:3405. PubMed
  29. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  30. Karandikar SH, et al. 2019. JCI Insight. 5. PubMed
  31. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  32. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  33. Bennion BG, et al. 2019. J Virol. 93. PubMed
  34. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  35. Schuster M, et al. 2017. J Immunol. 199:920. PubMed
  36. Schuster M, et al. 2019. Front Immunol. 10:1583. PubMed
  37. Highton AJ, et al. 2019. Wellcome Open Res. 4:78. PubMed
  38. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  39. Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
  40. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
  41. Tang Y, et al. 2019. Nat Commun. 10:4157. PubMed
  42. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
  43. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  44. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  45. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  46. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  47. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  48. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  49. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  50. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  51. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  52. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  53. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  54. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  55. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  56. Green DP et al. 2019. Neuron. 101(3):412-420 . PubMed
  57. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  58. Zhou J et al. 2019. Immunity. 50(2):403-417 . PubMed
  59. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  60. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  61. Yoon J et al. 2018. J Vis Exp. -141 . PubMed
  62. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  63. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  64. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  65. Parks CA, et al. 2019. Proc Natl Acad Sci U S A. 116:3136. PubMed
  66. Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed
  67. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  68. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  69. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  70. Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed
  71. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  72. Eckert EC, et al. 2020. Mol Ther Oncolytics. 0.710416667. PubMed
RRID
AB_2075239 (BioLegend Cat. No. 100733)
AB_2075238 (BioLegend Cat. No. 100734)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 10-23-2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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