FITC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
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109805 50 µg 44€
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109806 500 µg 93€
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  2. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  3. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  4. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  5. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  6. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  7. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  8. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  9. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  10. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  11. Burman A, et al. 2007. Blood. 110:1064. PubMed
  12. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  13. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  14. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  15. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  16. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  17. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  18. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  19. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  20. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  21. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  22. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  23. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  24. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  25. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  26. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  27. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  28. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  29. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  30. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  31. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  32. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  33. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  34. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  35. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  36. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  37. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  38. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  39. Li J, et al. 2020. Elife. 9:00. PubMed
  40. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  41. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  42. Wan X, et al. 2018. Nature. 560:107. PubMed
  43. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  44. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  45. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  46. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  47. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  48. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  49. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  50. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  51. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  52. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  53. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  54. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  55. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  56. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  57. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  58. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  59. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  60. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  61. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  62. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  63. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  64. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  65. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  66. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  67. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  68. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  69. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  70. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  71. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  72. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  73. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  74. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  75. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  76. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  77. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  78. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  79. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  80. Koide S, et al. 2022. iScience. 25:103603. PubMed
  81. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  82. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  83. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  84. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  85. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  86. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  87. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  88. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  89. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  90. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  91. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  92. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  93. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  94. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  95. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  96. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  97. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  98. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  99. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  100. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  101. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  102. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  103. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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